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pFLAG-ZYG11A

Plasmid

#110551

Purpose

expresses a FLAG-tagged human ZYG11A protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11A (ZYG11A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM7-C-19

Plasmid

#54173

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM7

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSUMO-LIC-CHIKV-nsP2h

Plasmid

#127935

Purpose

Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

Depositor

Dahai Luo

Article

Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

Insert

CHIKV nsP2h

Tags

His6-Sumo-TEV

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM5-C-19

Plasmid

#54169

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM5

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-MCM3-C-19

Plasmid

#54165

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mId1-Venus

Plasmid

#20966

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Inhibitor of DNA binding 1 (Id1 Mouse)

Tags

Venus

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pJB158

Plasmid

#202601

Purpose

RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

Depositor

Athanasios Typas

Article

Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-ALFA-tag-Gal4-VP16

Plasmid

#199465

Purpose

Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

Gal4-ALFA-tag-VP16

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMK401 (Hygro-P2A-mAID-Nluc)

Plasmid

#121196

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-Nluc

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_BE4_IVT

Plasmid

#171762

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

BE4

Use

Vector for in-vitro transcription

Promoter

T3 promoter

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-Calreticulin-N-10

Plasmid

#57353

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calreticulin (CALR Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46612

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-335-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_NR1H3_Hormone-recep

Plasmid

#109950

Purpose

Protein expression and purification of NR1H3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1H3_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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jk56

Plasmid

#87009

Purpose

genomic Sindbis virus plasmid for expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

Nrx1B
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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3xnls-G-Ca-FLITS

Plasmid

#191463

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

3xNLS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1D1_Hormone-recep

Plasmid

#109960

Purpose

Protein expression and purification of NR1D1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1D1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_NES_C1

Plasmid

#85062

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJG80

Plasmid

#89282

Purpose

Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2851

Plasmid

#87761

Purpose

Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG(Q103L)

Tags

PH

Expression

Worm

Promoter

Punc-17b

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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