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Plasmid#179398PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromoDepositorInsertdPC_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A5
Plasmid#130280PurposeCOL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor templateDepositorInsertmCherry-eGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A3
Plasmid#130279PurposeCOL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor templateDepositorInsertmCherry-eGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE792
Plasmid#167300PurposeVector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-TC
Plasmid#183754PurposeAAVS1 targeting construct with the Bxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox257
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_TAF3_2xPhd
Plasmid#179399PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhdDepositorInsertTAF3_2xPhd
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_MBD1_MBD
Plasmid#179404PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBDDepositorInsertMBD1_MBD
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_2xChromo
Plasmid#179396PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromoDepositorInsertCBX7_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationG113V in flexible linkerPromoterCAGGSAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo_TAF3_Phd
Plasmid#179400PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_PhdDepositorInsertCBX7_Chromo - TAF3_Phd
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_dPC_Chromo_TAF3_Phd
Plasmid#179403PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_PhdDepositorInsertdPC_Chromo - TAF3_Phd
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA390
Plasmid#215957PurposeFragmid fragment: (guide cassette) guide expression; positive control reporterDepositorHas ServiceCloning Grade DNAInsertU6_v1; sgEGFP_v1 [Sp]; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_2xChromo-W35A
Plasmid#179397PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)DepositorInsertCBX7_2xChromo(W35A)
UseTagsAviTag and EGFPExpressionMammalianMutationChromodomain W35APromoterCAGGSAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX1_2xChromo-W42A
Plasmid#179394PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)DepositorInsertCBX1_2xChromo(W42A)
UseTagsAviTag and EGFPExpressionMammalianMutationChromodomain W42APromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_MBD1_MBD-R22A
Plasmid#179405PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)DepositorInsertMBD1_MBD(R22A)
UseTagsAviTag and EGFPExpressionMammalianMutationMBD R22APromoterCAGGSAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo-W35A_TAF3_Phd
Plasmid#179401PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_PhdDepositorInsertCBX7_Chromo(W35A) - TAF3_Phd
UseTagsAviTag and EGFPExpressionMammalianMutationChromodomain W35APromoterCAGGSAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SOX17-NLS-tdTomato-EPG
Plasmid#210466PurposeDonor plasmid for knock-in NLS-tdTomato into the human SOX17 locusDepositorInsertSOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA
Plasmid#179402PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)DepositorInsertCBX7_Chromo - TAF3_Phd(DW890/891AA)
UseTagsAviTag and EGFPExpressionMammalianMutationPhd domain DW890/891AAPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only