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Addgene

We narrowed to 9,186 results for: sacs

Showing: 1201 - 1230 of 9186 results
  1. pTCW018

    Plasmid
    #83531
    Purpose
    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin
    Depositor
    Insert
    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pARO9/pFUS1J2
    Available since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pMV-TRP1

    Plasmid
    #65335
    Purpose
    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs
    Depositor
    Insert
    TRP1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. Gal4pBS3-Pleum

    Plasmid
    #60330
    Purpose
    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
    Depositor
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. Gal4pBS1-Pleum

    Plasmid
    #59993
    Purpose
    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
    Depositor
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pT040

    Plasmid
    #67640
    Purpose
    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette
    Depositor
    Insert
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. ZJOM43

    Plasmid
    #133652
    Purpose
    Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Insert
    p1k-VPS4-DuDre (VPS4 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Insert
    Sed1 (SED1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pJS288

    Plasmid
    #200714
    Purpose
    To express yeast Hxt1 hexose transporter in yeast
    Depositor
    Inserts
    PMA1 promoter (PMA1 Budding Yeast)
    Hxt1 (HXT1 Budding Yeast)
    ADH2 Ter (ADH2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    domesticated for MoClo cloning (removal of intern…
    Promoter
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. ZJOM113

    Plasmid
    #133666
    Purpose
    Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Insert
    p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pYZ145

    Plasmid
    #98405
    Purpose
    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Inserts
    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1201 - 1230 of 9186 results