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Showing: 2001 - 2050 of 18908 results

  12. pEMS1062

    Plasmid
    #29011
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Cre/EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    Feb. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    29011-plasmid-map-sequence-id-13417

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  14. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Article
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

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  16. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113700
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113700-plasmid-map-sequence-id-231558

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  17. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113687
    Purpose
    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113687-plasmid-map-sequence-id-231825

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  18. pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

    Plasmid
    #111392
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    ChETA-EYFP
    Use
    AAV
    Tags
    EYFP and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111392-plasmid-map-sequence-id-212257

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  21. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113688
    Purpose
    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113688-plasmid-map-sequence-id-231828

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  27. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  29. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113689
    Purpose
    SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113689-plasmid-map-sequence-id-231823

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  32. MultiMate-PE2 HEK3

    Plasmid
    #206274
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production, cre-a…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206274-plasmid-map-sequence-id-406650

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  33. pMMACE DEST CMV PE2

    Plasmid
    #206276
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2
    Use
    CRISPR; Recombinant baculovirus production, multi…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206276-plasmid-map-sequence-id-406406

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  42. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113701
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113701-plasmid-map-sequence-id-232036

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  43. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113699
    Purpose
    Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113699-plasmid-map-sequence-id-229551

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  44. AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

    Plasmid
    #186045
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8m
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186045-plasmid-map-sequence-id-370173

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  45. AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

    Plasmid
    #186044
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8f
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186044-plasmid-map-sequence-id-370163

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  46. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-alpha
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

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  47. RV-Syn-Dio-mVenus

    Plasmid
    #153204
    Purpose
    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the rat synapsin promoter
    Depositor
    Lynette Lim
    Insert
    membrane-tagged-Venus
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    rat synapsin
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    153204-plasmid-map-sequence-id-298905

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  48. AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

    Plasmid
    #186047
    Purpose
    AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    LifeAct-jGCaMP8f
    Use
    AAV
    Tags
    LifeAct-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186047-plasmid-map-sequence-id-370161

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  49. AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

    Plasmid
    #186048
    Purpose
    AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    LifeAct-jGCaMP8m
    Use
    AAV
    Tags
    LifeAct-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    186048-plasmid-map-sequence-id-370148

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  50. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

    Plasmid
    #186046
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8s
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186046-plasmid-map-sequence-id-370170

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Showing: 2001 - 2050 of 18908 results