We narrowed to 28,411 results for: REP

1. pPICZ A-Strep-Opa1 (SB251)

   Plasmid

   #227600

   Purpose

   Inducible expression of Strep-tagged Opa1 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Use

   Tags

   Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 253-960 only

   Promoter

   AOX1

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ A-MFN2-Strep (SB253)

   Plasmid

   #227602

   Purpose

   Inducible expression of Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   Twin-StrepTag, 10xHis

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRaf-IRES-pErk reporter

   Plasmid

   #226170

   Purpose

   Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)
   

   Use

   Tags

   3xFlag-tdMCP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPI4-Strep-SNAP-CD80

   Plasmid

   #223581

   Purpose

   Protein purification of human CD80 with Twin-Strep and SNAP tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD80 (CD80 Human)
   

   Use

   Tags

   Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPI4-Strep-SNAP-PD-L2-His10

   Plasmid

   #223578

   Purpose

   Protein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   PD-L2 (PDCD1LG2 Human)
   

   Use

   Tags

   His10 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

   Plasmid

   #217665

   Purpose

   Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   OsTir1 F74G-SNAP-Blast-AAVS1
   

   Use

   Tags

   SNAP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304-HsTtyh1-TwinStrep-His

   Plasmid

   #211332

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Tweety homology protein 1
   

   Use

   Lentiviral

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   W417R

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-HsProm1s1-TwinStrep-His

   Plasmid

   #210816

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJS-BCD-Strep-PCi

   Plasmid

   #214038

   Purpose

   Bacterial expression plasmid for strep-tagged Pci from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCi
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJS-BCD-Strep-PCaspase

    Plasmid

    #214034

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJS-BCD-Strep-dPCaspase

    Plasmid

    #214035

    Purpose

    Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    H79A, C146A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-Repo-Man-1-890

    Plasmid

    #184272

    Purpose

    Expresses C-terminally truncated GFP-RepoMan

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell division cycle associated 2 (CDCA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    truncated Repo-Man after aminoacid 893

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. STREP-SUMO-FLAG-SeKaiC

    Plasmid

    #218179

    Purpose

    WT SeKaiC for phosphorylation/dephosphorylation study

    Depositor

    Andy LiWang

    Article

    Kim et al Proc Natl Acad Sci U S A. 2008 Sep 2;105

    Insert

    STREP-SUMO-FLAG-SeKaiC
    

    Use

    Tags

    STREP-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG426-GAL-Prepro-3x-Adan

    Plasmid

    #181733

    Purpose

    Galactose inducible expression of Prepro-3x-Adan

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Adan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1-His6-E3(1)-CUbo-TwinStrep

    Plasmid

    #212712

    Purpose

    Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    6xHis and CUbo-TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFB MBD2a-2-strep (pc1794)

    Plasmid

    #211715

    Purpose

    Expresses fl. MBD2 2xStrep tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MBD2
    

    Use

    Tags

    2xStrep

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSF-DUET-C17orf53_1-87-Strep

    Plasmid

    #211433

    Purpose

    bacterial expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to a Strep-Tag

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Use

    Tags

    Strep-Tag

    Expression

    Bacterial

    Mutation

    Deletion of aa 88-646

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid

    #208644

    Purpose

    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.

    Depositor

    Quanfu Ma

    Insert

    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    

    Use

    CRISPR; Ssdna donnor preparation

    Tags

    Twin Strep

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-mEGFP(A206K)-TwStrep

    Plasmid

    #208622

    Purpose

    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone

    Depositor

    Quanfu Ma

    Insert

    mEGFP(A206K)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    (compared with pEGFP-C1, protein level) A206K

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFsynW SYT1-PGM reporter

    Plasmid

    #197278

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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