We narrowed to 65,871 results for: des.1

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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM4D(Gi)-mCherry

Plasmid

#44362

Purpose

Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

Depositor

Bryan Roth

Article

Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry

Use

AAV; Adeno associated viral vector

Tags

mCherry

Promoter

human Synapsin 1

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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SIRT2 Flag

Plasmid

#13813

Purpose

Mammalian expression of human SIRT2 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT2 (SIRT2 Human)

Tags

flag

Expression

Mammalian

Mutation

Transcript variant 2

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-VP64

Plasmid

#47107

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

Flag, HA, SV40 NLS, and VP64

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-C4-Nrf2

Plasmid

#21549

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

EGFP and Myc

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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EZ-Tet-pLKO-Puro

Plasmid

#85966

Purpose

Tet/Dox inducible shRNA lentivirus with puromycin selection

Depositor

Cindy Miranti

Article

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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VinculinTS

Plasmid

#26019

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Vinculin tension sensor

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

mpeg1.1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-F-del Casp9.IRES.GFP

Plasmid

#15567

Depositor

David Spencer

Article

Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

Insert

FKBP12(V36)-p30Caspase9 (FKBP1A Human)

Use

Retroviral

Tags

FKBP12 and HA epitope

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc DeltaN TCF4

Plasmid

#16513

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Expression

Mammalian

Mutation

Delta N: lacks aa 1-32

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
HDAC4 Flag

Plasmid

#13821

Purpose

Mammalian expression of human histone deacetylase 4 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC4 (HDAC4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
Gal4-PGC1 alpha

Plasmid

#8892

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
pMK243 (Tet-OsTIR1-PURO)

Plasmid

#72835

Purpose

Expresses OsTIR1 under the control of the Tet promoter

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

AAVS1-Tet-OsTIR1-PURO-AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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Pink Flamindo

Plasmid

#102356

Purpose

biosensor for cAMP in mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

Insert

Pink Flamindo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSIRV-NFAT-eGFP

Plasmid

#118031

Purpose

Fluorescent reporter for NFAT activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eGFP

Use

Retroviral

Promoter

minimal promoter with NFAT binding site

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Parkin

Plasmid

#23956

Purpose

Mammalian expression of human Park2 fused to mCherry

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Park2 (PRKN Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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rtTA-N144

Plasmid

#66810

Purpose

2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

Reverse tetracycline-controlled transactivator

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pK19pA-MN

Plasmid

#86973

Purpose

ChIC; genomic mapping of chromatin proteins

Depositor

Ulrich Laemmli

Article

Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

Insert

protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)

Expression

Bacterial

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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mPiezo1-IRES-eGFP

Plasmid

#80925

Purpose

expresses mouse Piezo1 with GFP

Depositor

Ardem Patapoutian

Article

Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

Insert

mouse Piezo1 CDS (Piezo1 Mouse)

Expression

Mammalian

Available Since

Nov. 25, 2016

Availability

Academic Institutions and Nonprofits only

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