We narrowed to 2,735 results for: ubiquitin

Showing: 801 - 820 of 2735 results

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pBMN mCherry-OPTN(D474N)

Plasmid

#119683

Purpose

For mammalian expression of UBD mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

D474N

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA3-TSC1

Plasmid

#19911

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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Level 1 P2 OsUbiP:Cas9:NosT

Plasmid

#165424

Purpose

GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Use

Synthetic Biology

Promoter

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPROEX-HTc-Flag3-Nrf2

Plasmid

#21553

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

6xHis and Flag3

Expression

Bacterial

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
p3XFlag-CMV10-Flag-mMcl-1

Plasmid

#32978

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 V5 hHR23A

Plasmid

#13053

Depositor

Steven Grossman

Article

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Insert

hHR23A (RAD23A Human)

Tags

His and V5

Expression

Mammalian

Mutation

R325K and L355P relative to NP_001257291.1

Available Since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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SENP2M497A

Plasmid

#61088

Purpose

cleave the SUMO off when use PETSUMO vector for express fusion protein

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

SENP2M497A catalytic domain (SENP2 Human)

Tags

His

Expression

Bacterial

Mutation

M497A; Catalytic domain is residues 364-589

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET-Duet1-His-TEV-mCherry-p62

Plasmid

#199780

Purpose

Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

6x His, TEV, mCherry

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFLAG-ZYG11B

Plasmid

#110550

Purpose

expresses a FLAG-tagged human ZYG11B protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11B (ZYG11B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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[UBC][NRE Pum][eGFP]

Plasmid

#97192

Purpose

The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

wild type Pumilio homology domain

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Promoter

UBC (ubiquitin C)

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
YFP-HA-DAT

Plasmid

#90244

Purpose

Expresses YFP-HA-DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

residues 193–203 of human DAT were replaced in YF…

Promoter

CMV

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-Flag-mMcl-1

Plasmid

#32982

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3-T7-Keap1

Plasmid

#21552

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

kelch-like ECH-associated protein 1 (KEAP1 Human)

Tags

T7

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-R4-uAb

Plasmid

#101800

Purpose

Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-FLAG

Plasmid

#86870

Purpose

To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3

Plasmid

#127128

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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