We narrowed to 16,582 results for: EMB

1. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

   Plasmid

   #220090

   Purpose

   Recruitable PIP5K to make PIP2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PIP5K1C (PIP5K1C Human)
   

   Use

   Tags

   TagBFP-FKBP1A(3-108)

   Expression

   Mammalian

   Mutation

   amino acids 1-635; D101R, R304D, R445E, K446E

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNeonGreen-C1-Nir1-LNS2

   Plasmid

   #220083

   Purpose

   high affinity PA biosensor with green fluorophore

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   NeonGreen-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Duet1-His-TEV-mCherry-p62

   Plasmid

   #199780

   Purpose

   Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   p62 (NUP62 Human)
   

   Use

   Tags

   6x His, TEV, mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xAP-1_RE::Renilla

   Plasmid

   #178317

   Purpose

   AP-1 Renilla luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   AP-1 Renilla Reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFLAG-Halo-Dyskerin

   Plasmid

   #207578

   Purpose

   CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag DKC1
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAGGS-MCS-NLS-3XFLAG-V5

   Plasmid

   #196383

   Purpose

   Empty backbone vector to insert cDNA for strong expression in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAGGs

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. UBXD1-GFP

   Plasmid

   #86464

   Purpose

   Expression of human UBXD1 with C-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   UBXD1 (UBXN6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-Tight-Puro_MYRF

   Plasmid

   #64843

   Purpose

   A lentiviral vector for the expression of mouse MYRF under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   MYRF (Myrf Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Rab4a-7

    Plasmid

    #56440

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pME-3x-FLAG-Ubiquitin (JDW 1098)

    Plasmid

    #224513

    Purpose

    A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3x-FLAG-Ubiquitin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. T/brachyury(2A-Dre)

    Plasmid

    #140761

    Purpose

    Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    Dre
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Endogenous T/brachyury

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcKv10.1

    Plasmid

    #85703

    Purpose

    mammalian expression Kv10.1

    Depositor

    Luis Pardo

    Article

    Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

    Insert

    KCNH1 (KCNH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/Myc-DNMT3B1

    Plasmid

    #35522

    Purpose

    Mammalian expression of human DNMT3B1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B1 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. IgK-IFN-luc

    Plasmid

    #14886

    Purpose

    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

    Depositor

    David Baltimore

    Article

    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

    Insert

    IgK-IFN (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. TB:7xE-Box:MiniP

    Plasmid

    #133880

    Purpose

    7 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies of the E-box motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HSC1-GiP

    Plasmid

    #58254

    Purpose

    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Mfn1-Myc

    Plasmid

    #23212

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn1 (Mfn1 Mouse)
    

    Use

    Tags

    6xHis, 9xMyc, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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