We narrowed to 13,964 results for: GAN

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pSL3936 (pDonor(AAVS1)_Pse)

Plasmid

#200903

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDIRECT_26G

Plasmid

#91149

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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Neurl1-Flag pcDNA3.1

Plasmid

#17282

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xFlag-MAPKAPK2

Plasmid

#82720

Purpose

Expresses human MAPKAPK2

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MAPKAPK2 (MAPKAPK2 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xFlag-STAT1

Plasmid

#82722

Purpose

Expresses human STAT1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

STAT1 (STAT1 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pRSF-G1(4CNW)RS

Plasmid

#222871

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-mScarlet

Plasmid

#131000

Purpose

AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

mScarlet

Use

AAV

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

Plasmid

#107939

Purpose

pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

mCherry(dpiRNA)::ANI-1(AHPH)

Expression

Worm

Promoter

pie-1

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-TAPP1-PH

Plasmid

#67662

Purpose

Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-TAPP1-PH

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS2

Plasmid

#128106

Purpose

Express HA tagged Nrxn1α-LNS2

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS2 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS4

Plasmid

#128108

Purpose

Express HA tagged Nrxn1α-LNS4

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS4 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS5

Plasmid

#128109

Purpose

Express HA tagged Nrxn1α-LNS5

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS5 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAB1679 CMV-HIVNES-GS-Cas13bt3

Plasmid

#176317

Purpose

CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt3

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-p3-PE(1153-dRnH)-C-term

Plasmid

#187182

Purpose

p3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1

Depositor

Gerald Schwank

Article

Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

Insert

PE2 from plasmid #132775 with shortened RnaseH domain

Use

AAV

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS2 (pBS0249)

Plasmid

#185139

Purpose

For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P202A/F203A)-FLAG

Plasmid

#164976

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P202A/ F203A

Promoter

EF1a

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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CMV BARK D110A p2A mCherry

Plasmid

#117690

Purpose

CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Tags

HA / FLAG

Expression

Mammalian

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Engrailed repressor domain (bases encoding amino …

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-14

Plasmid

#228994

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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