We narrowed to 12,153 results for: 112

1. pTrc99S-ssDsbA-PD-4xDQNAT

   Plasmid

   #128399

   Purpose

   Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   H. influenzae protein D
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTrc99S-ssDsbA-PorA-4xDQNAT

   Plasmid

   #128400

   Purpose

   Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   N. meningitidis PorA
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKD011 CMV 3xFLAG-NLS-ZF2-ABI1 in TUPV2

   Plasmid

   #161548

   Purpose

   Constitutive expression of 3xFLAG-NLS-ZF2-ABI1 under the CMV promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-ZF2-ABI1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

   Plasmid

   #149656

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2

   Plasmid

   #161543

   Purpose

   Constitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-VP64-ZF2
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

   Plasmid

   #161533

   Purpose

   Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-VP64-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-TTF-1-Q50K-Y54M

   Plasmid

   #75264

   Purpose

   Expresses a double point mutant of human TTF-1

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   TTF-1 (NKX2-1 Human)
   

   Tags

   none

   Expression

   Mammalian

   Mutation

   Q50K and Y54M in the homeodomain

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

   Plasmid

   #46839

   Purpose

   Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR_Hu_Plk5(WT)

   Plasmid

   #136329

   Purpose

   Human Plk5 WT in Gateway pENTR

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (PLK5 Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPNprM-hp

    Plasmid

    #48120

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of NprM
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. OphA-delta C6

    Plasmid

    #127679

    Purpose

    Bacterial expression of His-tagged OphA-delta C6

    Depositor

    James Naismith

    Article

    Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

    Insert

    OphA-delta C6
    

    Tags

    His8

    Expression

    Bacterial

    Mutation

    delta C6

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAL950

    Plasmid

    #119748

    Purpose

    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    Internal NdeI site removed and mutations Alanine …

    Promoter

    T7

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. UKNP1.19.1

    Plasmid

    #98192

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG5HA-MAGE-KA240,245

    Plasmid

    #89140

    Purpose

    mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429 with KA240,KA245 ubiquitination sites mutated

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K240A, K245A

    Promoter

    SV40

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

    Plasmid

    #46838

    Purpose

    Express cGFP transcript ending in the minimal functional MALAT1 triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZJOM8

    Plasmid

    #133671

    Purpose

    Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SNF7-GFP (SNF7 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-SHIV327C-HuA10-gp160

    Plasmid

    #123270

    Purpose

    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    SHIV327C-Hu A10 Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSPVpr-hp

    Plasmid

    #48119

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of Vpr
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOGG113

    Plasmid

    #133124

    Purpose

    pL0M-PU-NoP, promotorless region used as negative control

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    NoP promotorless region from plasmid pIJJ11268
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

    Plasmid

    #46840

    Purpose

    Express cGFP transcript ending in the wildtype MEN beta triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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