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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Article

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Tags

No tag

Expression

Mammalian

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-V5-AP-nes_pLX304

Plasmid

#120912

Purpose

Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

FKBP-V5-AP-NES

Use

Lentiviral

Expression

Mammalian

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMal-T-Avi-His/BirA

Plasmid

#102962

Purpose

Vector for in vivo biotinylation of a fusion protein in bacteria.

Depositor

Tonia Rex

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-LSD1

Plasmid

#92362

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-LSD1

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc. Inducible with tetracycline or doxycycline.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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BPK3082

Plasmid

#78742

Purpose

Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

LbCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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