We narrowed to 28,941 results for: Tat
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Plasmid#120400PurposeExpresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.DepositorInsertMutant form of Perfringolysin O
TagsHis6 tagExpressionBacterialMutationAmino acids 1-27 are deleted, starts at amino aci…Available SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tagged-IRES
Plasmid#35570DepositorInsertLymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
UseLuciferaseTagsFirefly Luciferase and MS2 Stem LoopExpressionMammalianPromoterSV40Available SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5/FRT/TO HIS HSPA1A
Plasmid#19537DepositorAvailable SinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-G SCT-T2A-GFP
Plasmid#205462Purposelentiviral plasmid for expression of HLA-G SCTDepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ELF1_pet28a
Plasmid#131649PurposeBacterial expression of ETS gene ELF1DepositorAvailable SinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAluYa5-neo-TET
Plasmid#51283Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertAluYa5
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterupstream pol III enhancer sequence of the 7SL gen…Available SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
Plasmid#115684PurposeNanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.DepositorInsertNluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
UseLentiviralAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Rheb-N153T
Plasmid#19997DepositorInsertRas homolog enriched in brain 1 - N153T (RHEB Human)
TagsFLAGExpressionMammalianMutationChanged Asparagine 153 to ThreonineAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v4
Plasmid#158032Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBV65
Plasmid#108363PurposeA nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.DepositorInsertloxP-natMX6-CgMET3prom-cre-loxP
UseGene disruption marker for candida glabrataPromoterC. glabrata MET3Available SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT8-mGold
Plasmid#183989PurposeExpresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.DepositorAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-mRuby3-FTH1
Plasmid#192804PurposeStable expression of mRuby3-FTH1 in mammalian cellsDepositorAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-cofilin WT
Plasmid#78295PurposeMammalian expression of mouse cofilin fused to EGFPDepositorAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1-p2A-eGFP
Plasmid#158699PurposeMammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection markerDepositorInsertscFLARE1-p2A-eGFP
UseAAVTagsFlag, V5. eGFPExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_WT-talin1 (1-2541)
Plasmid#166112PurposeFor expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.DepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PMXS-SLC1A3
Plasmid#72873PurposeThe retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson AssemblyDepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP T2A Cre
Plasmid#63704PurposeInducible expression of GFP T2A Cre fusion in mammalian cellsDepositorInsertT2A Cre
UseRetroviralTagsEGFPExpressionMammalianPromotertight TRE promoterAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
BRCA1 A10.2 gRNA
Plasmid#90549Purpose3rd generation lentiviral gRNA plasmid targeting human BRCA1DepositorInsertBRCA1 (Guide Designation A10.2)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE
Plasmid#214265PurposeExpression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.DepositorInsertFlag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
UseAAVTagsEGFPExpressionMammalianMutationN/APromoterSynapsinAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only