We narrowed to 8,924 results for: pcDNA3

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PA-RL-BAD

Plasmid

#78859

Purpose

DULIP positive control (bait) for assay establishment

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

BCL2 associated agonist of cell death (BAD Human)

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA HA-Gnat1 WT-3UTR

Plasmid

#184026

Purpose

Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 1

Plasmid

#135357

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-nSpyCas9 aa 1029

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-GPR150

Plasmid

#106050

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR150 (GPR150 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJO427

Plasmid

#111139

Purpose

URT-type vector for Glu-mIκBα expression in Mammalian cells

Depositor

Alexander Varshavsky

Article

Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-MED31

Plasmid

#15427

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
CFP-GIV-CT WT

Plasmid

#69532

Purpose

Mammalian expression of The CFP-tagged GIV-CT biosensors

Depositor

Pradipta Ghosh

Article

Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

Insert

GIV (CCDC88A Human)

Tags

CFP

Expression

Mammalian

Mutation

C terminal amino acids S1660-S1870 of human GIV

Promoter

CMV

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTE4565

Plasmid

#88903

Purpose

Expresses inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

Plasmid

#166809

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1322R

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG mTOR ST

Plasmid

#22999

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

mTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2035T

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pJHG004

Plasmid

#126455

Purpose

Expresses dTdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

dTdT (DNTT Human)

Expression

Mammalian

Mutation

Added D343E and D345E to inactivate the enzyme.

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR17

Plasmid

#106005

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR17; Opto-GPR17; A7)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR17 (GPR17 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pJO428

Plasmid

#111141

Purpose

URT-type vector for Val-mIκBα expression in Mammalian cells

Depositor

Alexander Varshavsky

Article

Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-hB90

Plasmid

#18968

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

Insert

Brevican C-terminus (BCAN Human)

Expression

Mammalian

Mutation

Human brevican signal peptide aa 1-23 followed by…

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pTE4567

Plasmid

#107557

Purpose

Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(dead2)

Tags

3xHA and NLS

Expression

Mammalian

Mutation

E1080A

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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Opto-GPR19

Plasmid

#106007

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR19 (GPR19 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL681 CHYRON4 integration construct

Plasmid

#126449

Purpose

To integrate the CHYRON4 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON4 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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HA-human-BNIP3 delta Exon2

Plasmid

#100782

Purpose

Mammalian expression of BNIP3 delta Exon2 splice variant

Depositor

Joseph Gordon

Article

Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

Insert

human-BNIP3 delta Exon2 (BNIP3 Human)

Tags

HA

Expression

Mammalian

Mutation

Exon2 deletion

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRD397

Plasmid

#163104

Purpose

Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mTurquoise2_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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