We narrowed to 22,439 results for: scr

1. mCarmine in pRSETB

   Plasmid

   #109484

   Purpose

   bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

   Depositor

   Oliver Griesbeck

   Article

   Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

   Insert

   mCarmine
   

   Use

   Tags

   N-terminal histidine tag in vector

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT11

   Plasmid

   #223383

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQAT08

   Plasmid

   #223380

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. QUAS-0-T7-GFP_ColE1

   Plasmid

   #171649

   Purpose

   QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-0-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. QUAS-10-T7-GFP_ColE1

   Plasmid

   #171654

   Purpose

   QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-10-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. QUAS-15-T7-GFP_ColE1

   Plasmid

   #171655

   Purpose

   QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-15-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDAC565

   Plasmid

   #195242

   Purpose

   Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-Cas9

   Plasmid

   #50661

   Purpose

   Doxycycline-inducible lentiviral expression of SpCas9

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-300(HAT-)

   Plasmid

   #23254

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H1415A E1423A Y1424A L1428S Y1430A H1434A

   Promoter

   Available since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRE-mCherry

    Plasmid

    #92202

    Purpose

    Fluorescent protein reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol-Promoter:NLS-tdMCP-eGFP

    Plasmid

    #86248

    Purpose

    For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Insert

    beta Actin promoter:NLS-tdMCPeGFP
    

    Use

    Used with transposase to make transient or stable…

    Tags

    NLS-tdMCP-eGFP

    Expression

    Mutation

    Promoter

    Beta Actin

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQAT10

    Plasmid

    #223382

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQAT09

    Plasmid

    #223381

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 phiC31

    Plasmid

    #68310

    Purpose

    In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

    Depositor

    Konrad Basler

    Article

    Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

    Insert

    phiC31
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGreen2x35SLUC

    Plasmid

    #108228

    Purpose

    Plant expression as translational fusions to FLUC with RLUC normalization

    Depositor

    Alejandro Ferrando

    Article

    Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge