We narrowed to 14,640 results for: RING

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pAB078d9

Plasmid

#79207

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK509

Plasmid

#110610

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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CMV+ plasmid

Plasmid

#169740

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK615

Plasmid

#110609

Purpose

BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

sgRNA

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pARF1-YFP

Plasmid

#11390

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

YFP

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pBTK121

Plasmid

#110581

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

PA3 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TB:7xSMAD_RE:MiniP

Plasmid

#133879

Purpose

7 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies the SMAD DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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p168

Plasmid

#62618

Purpose

ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSR02

Plasmid

#69149

Purpose

Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFLEX-m-cyclin D1 -T286A mutant

Plasmid

#86792

Purpose

mammalian expression of cyclin D1 T286A mutant

Depositor

Martine Roussel, Charles Sherr

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D1 (Ccnd1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T286A

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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POT2-RFP

Plasmid

#65318

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBLO2 casX

Plasmid

#123122

Purpose

E. coli expression vector for CasX sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX guide RNA

Use

CRISPR

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSin-5HT-1.0

Plasmid

#172882

Purpose

make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells

Depositor

J. Julius Zhu

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Insert

5HT SENSOR

Expression

Mammalian

Promoter

sp6

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

Plasmid

#32483

Purpose

Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

ChR2HA-2a-PSAML141F,Y115F:GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

ChR2 2A

Promoter

CAG

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMOD_A0402

Plasmid

#91009

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMSP1D1G1

Plasmid

#97325

Purpose

Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

Depositor

Michael Marty

Article

Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

Insert

MSP1D1G1

Tags

6xHis - TEV

Expression

Bacterial

Mutation

Addition of G residue after T25

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(KD)-mCherry

Plasmid

#87358

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

D388R

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-eGFP-dspB(E184Q)-6xHis

Plasmid

#175783

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag and eGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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p413TEF-SirT1

Plasmid

#51746

Purpose

overexpression of human SirT1 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

SirT1 (SIRT1 Human)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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Hmga2 3'UTR m4 luciferase (Luc-m4)

Plasmid

#14787

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-m4 (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

m4 mutant (see article and author's map for …

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only