We narrowed to 9,182 results for: Pol

1. pFastBac1-Ls-AChBP

   Plasmid

   #63126

   Purpose

   Expression of Lymnaea stagnalis AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSNAP-CLC-EYFP

   Plasmid

   #38011

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Tags

   EYFP and SNAP26m

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-T3-CjCAS9-pA

   Plasmid

   #131543

   Purpose

   Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   FLAG-SV40NLS, SV40NLS, and polyA

   Expression

   Mammalian

   Mutation

   Human codon-optimized

   Promoter

   CAG, T3

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSmart-EF1a-Nla-cas5

   Plasmid

   #178878

   Purpose

   Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas5c-bGH polyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSmart-EF1a-Nla-cas8

   Plasmid

   #178880

   Purpose

   Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas8c-bGH PolyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSmart-EF1a-Nla-cas7

   Plasmid

   #178879

   Purpose

   Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas7c-bGH PolyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-OSF-TRIM5alpha(human)

   Plasmid

   #79034

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human) (TRIM5 Human)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB

   Plasmid

   #162699

   Purpose

   Expresses pHnCB10 derived carboxysome operon and prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   pHnCB10 derived carboxysome operon, prk
   

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWPI/hPLK2K111M/Neo

    Plasmid

    #35386

    Depositor

    Anurag Tandon

    Article

    Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

    Insert

    hPLK2 K111M (PLK2 Human)
    

    Use

    Lentiviral

    Tags

    EMCV IRES-Neomycin cassette and V5 epitope and Po…

    Expression

    Mammalian

    Mutation

    K111M

    Promoter

    EF1-alpha

    Available Since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

12. AA302

    Plasmid

    #216052

    Purpose

    Fragmid fragment: (C' terminus) Nucleoplasmin NLS

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NpNLS_v1.2; Stop; polyA_v1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mRFP-LAP2β

    Plasmid

    #228029

    Purpose

    lentiviral construct for mRFP-LAP2β expression

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    LAP2b (Tmpo Rat)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFE-Rs-FI-prk

    Plasmid

    #162697

    Purpose

    Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    R. spaeroides Form IC rubisco and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-sod1

    Plasmid

    #107594

    Purpose

    Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    sod1 (sod1 Zebrafish)
    

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYFP-NEAT1m_v1-RT

    Plasmid

    #97089

    Purpose

    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    NEAT1m_v1-RT (NEAT1 Human)
    

    Use

    CRISPR

    Tags

    EYFP

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. IL2 pAcGP67a

    Plasmid

    #41806

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Tags

    6x His

    Expression

    Insect

    Promoter

    Polyhedron

    Available Since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pYFP-NEAT1_IS_RT

    Plasmid

    #97091

    Purpose

    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    NEAT1_IS_RT (NEAT1 Human)
    

    Use

    CRISPR

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. T2A-mCherry (MTK 4a) (pAN3844)

    Plasmid

    #194223

    Purpose

    Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    T2A-mCherry
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EYFP-Clathrin

    Plasmid

    #20921

    Depositor

    Xiaowei Zhuang

    Article

    Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

    Insert

    Clathrin, light polypeptide (Lca) (Clta Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only