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pTRE/SP M1WT

Plasmid

#89327

Purpose

Inducible expression of WT M1 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

Tet-responsive P tight

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 WT

Plasmid

#89328

Purpose

Inducible expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

Tet-responsive P tight

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_U2AF1_RRM

Plasmid

#110033

Purpose

Protein expression and purification of U2AF1_RRM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

U2AF1_RRM (U2AF1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NXT1_NTF2

Plasmid

#109803

Purpose

Protein expression and purification of NXT1_NTF2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NXT1_NTF2 (NXT1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2360

Plasmid

#36773

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX8-TALEN_Left (Cbx8 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
Dendra2-Zyxin-6

Plasmid

#57750

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Zyxin (ZYX Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry-miniSOG-Lifeact-7

Plasmid

#54846

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Lifeact-PAmCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_VCL_Vinculin

Plasmid

#110045

Purpose

Protein expression and purification of VCL_Vinculin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCL_Vinculin (VCL Human)

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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Dom34+GST

Plasmid

#19099

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag, GST, and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
LacD.1_WT_pET21a

Plasmid

#29789

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))

Tags

His

Expression

Bacterial

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
LacD_L165E/L275S_pET21a

Plasmid

#29788

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L165E and L275S

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
Tip alpha (21-192)

Plasmid

#23157

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
Ta1098

Plasmid

#19072

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pWRKY70::LUC

Plasmid

#141171

Purpose

Luminescent reporter for salicylic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtWRKY70 promoter

Use

Luciferase

Expression

Plant

Promoter

AtWRKY70, AT3G56400

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Clover-CytERM-N-17

Plasmid

#56301

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CytERM

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TAL2292

Plasmid

#36725

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ALK-TALEN_Left (Alk Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pHH0103_TOM1L2_VHS

Plasmid

#109901

Purpose

Protein expression and purification of TOM1L2_VHS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TOM1L2_VHS (TOM1L2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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907K

Plasmid

#160236

Purpose

FlightN-PipR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

pristinamycin repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCALNL-BsaI-EGFP

Plasmid

#123136

Purpose

Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

EGFP, mRFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

cBA

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBT100-neo

Plasmid

#123137

Purpose

Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Neomycin, poly-A terminator

Use

Synthetic Biology; Golden gate donor vector

Promoter

none

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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