We narrowed to 12,947 results for: 112
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Plasmid#134169PurposeBacterial expression of RB3 stathmin-like domain (SLD)DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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mOtop1_pGEMHE
Plasmid#114674PurposeThis construct was used to express mouse Otop1 in Xenopus laevis oocytesDepositorInsertOtopetrin 1 (Otop1 Mouse)
UseFor generating the mrna, which can highly express…MutationSNP (rs49325632): Glycine 396 to AlaninePromoterT7Available SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM25
Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH4542
Plasmid#200779PurposePtwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptuneDepositorInsertGCaMP6
TagsmNeptuneExpressionWormPromoterPtwk-40Available SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_3xFLAG_BIRC6_BIRmut
Plasmid#197968PurposeMammalian cell expression of 3xFLAG-BIRC6_BIRmutDepositorAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-CenpB 1-166
Plasmid#108475Purposeexpression of GFP-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
TagsEGFPExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
PU.1-ets-IRES-mCherry
Plasmid#80141Purposeretroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherryDepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationets = PU.1 DNA binding domain (amino acids 159-26…Available SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense
Plasmid#46835PurposeExpress cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZJOM103
Plasmid#133664PurposeIntegration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM96
Plasmid#133663PurposeIntegration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRCP
Plasmid#195862PurposeEncodes chromogenic protein (CP). CP inducible with rhamnose.DepositorInsertChromogenic Protein
ExpressionBacterialAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pb459-mU6-sgRNA-EF1a-PuroR
Plasmid#195507Purposepiggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sitesDepositorInsertPuromycin
UseCRISPR; PiggybacExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1 tdTomato toehold switch
Plasmid#107354PurposeExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressedDepositorInserttoehold switch 5 + tdTomato
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM195A
Plasmid#218125PurposeHybrid circuit displaying hsCRBN-CTD bait and driving expression of gIIIDepositorInsertsCRBN_CTD
M13 pIII
Tags434cI_RR69Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM194A
Plasmid#218124PurposeHybrid circuit displaying hsCRBN-CTD bait and driving expression of luxABDepositorInsertsCRBN_CTD
luxAB
Tags434cI_RR69Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA15-Ubc-NLS- scFV-tdTomato
Plasmid#199446PurposeExpression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cellsDepositorInsertscFV-tdTomato
UseLentiviralExpressionMammalianPromoterhuman ubiquitin C (UBC)Available SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-RhoB Sensor-IRES-Blast
Plasmid#222633PurposeRho reporterDepositorInsertRhoB Sensor
UseLentiviralPromoterEF1aAvailable SinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(K433E)
Plasmid#42514DepositorInsertPyruvate Kinase M2 Lys433->Glu
UseRetroviralTagsFlagMutationChanged lysine 433 to glutamateAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only