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pHAGE-CMV-Luc2-IRES-ZsGreen-W

Plasmid

#164432

Purpose

Lentiviral vector backbone encoding firefly luciferase and ZsGreen

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Inserts

Luciferase
ZsGreen

Use

Lentiviral

Available Since

Feb. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-565

Plasmid

#11520

Purpose

Gateway destination vector for bacterial expression; His,GST tag

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway Cloning

Tags

6xHis and GST

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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ABE-NW1

Plasmid

#240615

Purpose

Adenine base editor with narrowed editing window

Depositor

Tingting Jiang

Article

Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

Insert

TadA8e(N108Q,M151C,Q154Y)-linker-nCas9 (D10A)

Use

CRISPR

Expression

Mammalian

Mutation

TadA8e(N108Q,M151C,Q154Y),Cas9 (D10A)

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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Lifeact-mScarlet3-S2

Plasmid

#246873

Purpose

Expression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging actin filaments.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Lifeact

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-mTagBFP2

Plasmid

#34632

Depositor

Vladislav Verkhusha

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

mTagBFP2

Expression

Bacterial

Mutation

I174A compared to mTagBFP (enhanced photostabilit…

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Article

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-7xHis-TEV-hexa

Plasmid

#222868

Purpose

Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

Depositor

Nikolaus Loening

Article

Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

Insert

TEV protease

Tags

7xHis and MBP

Expression

Bacterial

Mutation

C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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