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We narrowed to 4,407 results for: crispr c plasmids

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Showing: 1231 - 1260 of 4407 results

  4. pAN057

    Plasmid
    #220053
    Purpose
    NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).
    Depositor
    Blake Wiedenheft
    Publication
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
    Expression
    Mammalian
    Mutation
    c.3846G>A mutation
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220053-plasmid-map-sequence-id-434400

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  7. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Publication
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    229676-plasmid-map-sequence-id-457076

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  8. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid
    #229678
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Publication
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    monomeric StayGold, HA
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    229678-plasmid-map-sequence-id-457125

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  11. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid
    #114093
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114093-plasmid-map-sequence-id-230492

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  14. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

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  15. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  16. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  26. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  27. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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Showing: 1231 - 1260 of 4407 results