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Plasmid#61513PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TA mutation (see comments), Tom20, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP
Plasmid#61512PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP, Tom20, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKalpha-KM
Plasmid#15468DepositorInsertIkB kinase alpha-KM (Chuk Mouse)
UseTagsFlagExpressionMammalianMutationchanged Lysine at 44 to AlaninePromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKbeta-KM
Plasmid#15466DepositorInsertIkB kinase beta-KM (Ikbkb Mouse)
UseTagsFlagExpressionMammalianMutationchanged Lysine at 44 to AlaninePromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Myc-IKKalpha
Plasmid#19739DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TE)
Plasmid#61522PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments), Tom20, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjACTB-EGFP-5'3'short
Plasmid#129750PurposeGene targeting in marmoset cellsDepositorInsertACTB (ACTB C. jacchus (common marmost))
UseMarmoset targetingTagsEGFPExpressionMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR-HA-IKKalphad17
Plasmid#19738DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsHAExpressionMammalianMutationSplice variant lacking 17 amino acids at the C-te…PromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)
Plasmid#223140PurposeExpression of truncated HP1a with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 72-177PromoterEF1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)
Plasmid#223145PurposeExpression of truncated MBD2 with dCjCas9 and empty gRNA scaffoldDepositorInsertMBD2 (MBD2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 176-231PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)
Plasmid#223150PurposeExpression of truncated HP1a with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 72-177PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)
Plasmid#223144PurposeExpression of truncated MBD1 with dCjCas9 and empty gRNA scaffoldDepositorInsertMBD1 (MBD1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 529-592PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)
Plasmid#223154PurposeExpression of truncated MBD1 with dSaCas9 and empty gRNA scaffoldDepositorInsertMBD1 (MBD1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 529-592PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)
Plasmid#223155PurposeExpression of truncated MBD2 with dSaCas9 and empty gRNA scaffoldDepositorInsertMBD2 (MBD2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 176-231PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223159PurposeExpression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2
Plasmid#223149PurposeExpression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)
Plasmid#223148PurposeExpression of truncated MECP2 with dCjCas9 and empty gRNA scaffoldDepositorInsertMECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310PromoterEF1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TD)
Plasmid#61521PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TD mutation (see comments), Tom20, and m…ExpressionMammalianMutationTD mutation (see comments)PromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)
Plasmid#223142PurposeExpression of truncated HP1b with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 79-173PromoterEF1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)
Plasmid#223151PurposeExpression of truncated HP1a with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 115-177PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)
Plasmid#223141PurposeExpression of truncated HP1a with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 115-177PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)
Plasmid#223158PurposeExpression of truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertMECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)
Plasmid#223152PurposeExpression of truncated HP1b with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 79-173PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223164Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/CD63mScarlet
Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorInsertCD63-mScarlet (CD63 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
AIP(WT)-mChF-giantin
Plasmid#61523PurposeExpression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIPDepositorUseTagsAIP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBK2045-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(CMV-gRNA1)
Plasmid#223165Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting CMVDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)
Plasmid#223167Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)
Plasmid#223162PurposeExpression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)
Plasmid#223143PurposeExpression of truncated HP1b with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 111-173PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)
Plasmid#223153PurposeExpression of truncated HP1b with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 111-173PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)
Plasmid#223161PurposeExpression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)
Plasmid#223166Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-smGFP-Kv2.1
Plasmid#131709PurposeExpresses Kv2.1 in mammalian cellsDepositorInsertKCNB1 (KCNB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-codon optimized catalytically inactive PHGDH
Plasmid#154903PurposeExpresses codon optimized catalytically inactive PHGDH in mammalian cellsDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationThree mutations: D175N, R236K, H283APromoterTREAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI30
Plasmid#183749PurposeAAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.DepositorInsertAAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
UseAAVTagsExpressionMutationK449R, and NNSTRGG inserted between 588 and 589 o…Promoterp41Available sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT309 RapInducible1-ActivatorCloneSite-Citrine
Plasmid#187321PurposeRapamycin inducible genetic circuit with cloning site for activator domainsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA324
Plasmid#215953PurposeFragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
C76m
Plasmid#120988PurposeMoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertKanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPD47_52
Plasmid#1446DepositorTypeEmpty backboneUseTagsSp6p, T7p, OriLam>>, OriLam<<, KanRN,…ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pHflu3
Plasmid#203881PurposepHI1 with insertion of kanamycin-resistance geneDepositorInsertaminoglycoside O-phosphotransferase APH(3')-IIa
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSG360
Plasmid#27155DepositorTypeEmpty backboneUseMycobacterial ko vectorTagsExpressionMutationPromoterAvailable sinceMarch 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSG360zeo
Plasmid#27154DepositorTypeEmpty backboneUseMycobacterial ko vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDB88
Plasmid#26958DepositorInserte. coli galactokinase
UseTagsExpressionBacterialMutationcloned under the MOP promoterPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1
Plasmid#32022DepositorInsertDCR1
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC
Plasmid#32023DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1(1-384)
Plasmid#32031DepositorInsertDCR1(1-384)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaNdeltaC
Plasmid#32030DepositorInsertDCR1(107-355)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only