We narrowed to 13,317 results for: Mpl;

1. pET28b(+)-SPN+IFS

   Plasmid

   #83372

   Purpose

   Expresses SPN and IFS complex in E. coli cell

   Depositor

   SeWon Suh

   Article

   Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

   Inserts

   SPN
   IFS
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXW117As(RS-E11)

   Plasmid

   #123145

   Purpose

   Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J117, ParsR, PhrpLE, Pecf11

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Arp3_pLib

   Plasmid

   #173677

   Purpose

   Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   Arp3 (ACTR3 Human)
   

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPEY-Z3-Nat-ADE2

   Plasmid

   #232106

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPEY-GAL-Hyg-ADE2

   Plasmid

   #232104

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPEY-Z3-Hyg-ADE2

   Plasmid

   #232107

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pToxAmp12

   Plasmid

   #218286

   Purpose

   Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-RPLP15UTR-hRluc-A60

   Plasmid

   #182639

   Purpose

   Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

   Depositor

   Stephen Floor

   Article

   Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

   Insert

   Renilla luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The transcription is driven by a T7 promoter, pai…

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

   Plasmid

   #199447

   Purpose

   Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   dSaCas9-10xGCN4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human ubiquitin C (UBC)

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1.Gαq-LgB97

    Plasmid

    #134360

    Purpose

    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαq-LgB97 (GNAQ Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXW109Hg(RS-RinA-E11)2

    Plasmid

    #123151

    Purpose

    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1.Gαi1-LgB91

    Plasmid

    #134342

    Purpose

    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi1-LgB91 (GNAI1 Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_Thy1StTA

    Plasmid

    #97411

    Purpose

    TET driver for amplified expression in cortical neurons

    Depositor

    Tetsuo Yamamori

    Article

    Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mouse thy1PSs

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

    Plasmid

    #159817

    Purpose

    Positive control for germline GFP expression from simple array in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXW117As(RS-E11-RinA)2

    Plasmid

    #123146

    Purpose

    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fireworks RFP[PTC+] (AVA2626)

    Plasmid

    #85444

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-sACt-mCherry-NES

    Plasmid

    #138215

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NES (Adcy10 Rat)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Fireworks GFP[PTC+] (AVA2600)

    Plasmid

    #85446

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRL838

    Plasmid

    #70680

    Purpose

    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

    Depositor

    Peter Wolk

    Article

    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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