We narrowed to 9,934 results for: Uty

1. pAG109 H2B-FAST

   Plasmid

   #130722

   Purpose

   Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   H2B-FAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-β2AR-μDpTT

   Plasmid

   #182326

   Purpose

   Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
   

   Use

   Tags

   Full-length human beta2 adrenergic receptor

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-RIIα-μDpTT

   Plasmid

   #182327

   Purpose

   PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
   

   Use

   Tags

   Full-length human PKA type 2alpha regulatory subu…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVAX1-BMP2/7 -

   Plasmid

   #137912

   Purpose

   Constitutive mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)

   Depositor

   Georg Feichtinger

   Article

   Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

   Inserts

   BMP2 (BMP2 Human)
   BMP7 (BMP7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lyn-FLINC-EKAR1

   Plasmid

   #87707

   Purpose

   Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   Lyn-FLINC-EKAR1
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBrain-GFP-TACC3KDP∆678-681-shTACC3

   Plasmid

   #69115

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of 678-681aa region. Silent mutations i…

   Promoter

   CMV

   Available Since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-MmCENPT-4NS-GFP

   Plasmid

   #237431

   Purpose

   Expresses Mus musculus CENP-T with 4 rat-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with 4 rat-specific residues in HFD (Cenpt Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   432 T to A; 463 I to T, 470 L to E, 485 Q to L

   Promoter

   T7

   Available Since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gfa104-hIRβ

   Plasmid

   #236227

   Purpose

   Expressing a truncated, constitutively active human insulin receptor (IRβ) in astrocyte

   Depositor

   Olivier Thibault

   Article

   Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

   Insert

   human Insulin Receptor beta subunit (INSR Human)
   

   Use

   AAV

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

   Plasmid

   #218463

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lys1269Ala and His1271Ala substitutions

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBT9-Hook1

    Plasmid

    #198523

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook1 (HOOK1 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent substitution in codon 620 to eliminate int…

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVone-AAV-PHP.eB-CMV-eGFP

    Plasmid

    #230929

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVone-AAV9-CMV-eGFP

    Plasmid

    #230928

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-SFFV-GLuc-CreERT2-mCherry

    Plasmid

    #178185

    Purpose

    the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.

    Depositor

    Irmela Jeremias

    Article

    Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:

    Inserts

    GLuc
    CreERT2
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVone-AAV2-CMV-eGFP

    Plasmid

    #230930

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBrain-ch-TOGKDP-GFP-shch-TOG

    Plasmid

    #69113

    Purpose

    Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Silent mutations to confer shRNA resistance.

    Promoter

    CMV

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV Puro DEST (w118-1) eGFP

    Plasmid

    #107505

    Purpose

    Expresses eGFP, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PKAsub-TagRFPT-CAAX

    Plasmid

    #87712

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    PKAsub-TagRFPT-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV Puro DEST (w118-1) FITM2

    Plasmid

    #107504

    Purpose

    Expresses FITM2, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    FITM2 (codon optimized)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid

    #85821

    Purpose

    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    Zf Ubi::cMyc oDam_f_GFPoNLS
    

    Use

    Tol2 based vector

    Tags

    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

    Expression

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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