We narrowed to 63,651 results for: cloning

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Flag-HA-USP8

Plasmid

#22608

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP8 (USP8 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Publication

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Publication

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

71 Articles

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pcDNA3.2/v5-DEST hGlut1

Plasmid

#18085

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Glut1 (SLC2A1 Human)

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEF5B-FRT-GFP-αTAT1

Plasmid

#27099

Depositor

Maxence Nachury

Publication

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

α-Tubulin K40 acetyltransferase1 (ATAT1 Human)

Tags

GFP, S-tag, and TEV site

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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Flag-HA-GFP

Plasmid

#22612

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pLenti CMV/TO Zeo DEST (644-1)

Plasmid

#17294

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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JAK2 (V617F)-pcw107-V5

Plasmid

#64610

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

JAK2 (JAK2 Human)

Use

Lentiviral

Tags

V5

Mutation

V617F

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGADT7-GW

Plasmid

#61702

Purpose

Gateway compatible Y2H vector - AD

Depositor

Yuhai Cui

Publication

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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Flag-HA-USP14

Plasmid

#22569

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP14 (USP14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCW57-CMV-ssRFP-GFP-KDEL

Plasmid

#128257

Purpose

Fluorescence reporter to monitor ER-phagy activity

Depositor

Noboru Mizushima

Publication

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

ssRFPGFPKDEL

Use

Lentiviral

Tags

RFPGFP

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Flag-HA-BAP1

Plasmid

#22539

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

Plasmid

#139998

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

Promoter

CAG

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

Encodes anti-HA scFv (anti-HA frankenbody) fused with mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pET-Cas9-6xHis

Plasmid

#62374

Purpose

Expression of Cas9-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9_6xHis

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Publication

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pT3-N90-beta-catenin

Plasmid

#31785

Depositor

Xin Chen

Publication

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

beta-catenin (CTNNB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of aa 1-90- Constitutitvely Active

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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