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Plasmid#115171PurposeExpresses membrane-targeted Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmembrane targeting sequence and mycExpressionMammalianMutationFirst 32 amino acids of Sema4A replaced with memb…PromoterCMVAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TC1464
Plasmid#164881PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1DepositorInsertdcas13d-ADARdd
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAL-MajSat-FLAG-NLS-MCS
Plasmid#69073PurposeExpresses TALE for mouse major satellite with FLAG tag under CAG promoterDepositorInsertTALE
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 13
Plasmid#36110DepositorInsertG protein subunit Gamma 13 (GNG13 )
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 12
Plasmid#36109DepositorInsertG protein subunit Gamma 12 (Gng12 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pOmpT
Plasmid#112123PurposeBacterial expression outer membrane protein T (OmpT), wild typeDepositorInsertOmpT (ompT E. coli)
UseTagsExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOmpW
Plasmid#112121PurposeBacterial expression of outer membrane protein W (OmpW), wild typeDepositorInsertOmpW (ompW E. coli)
UseTagsExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 7
Plasmid#36105DepositorInsertG protein subunit Gamma 7 (GNG7 Bovine)
UseTagsYFPExpressionMammalianMutationHIs tag between YFP and insertPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 4
Plasmid#36104DepositorInsertG protein subunit Gamma 4 (Gng4 Mouse)
UseTagsYFPExpressionMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
UseTagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_AKAR5’
Plasmid#114141PurposeMammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5'
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PADH1-TIR1-9myc-URA3
Plasmid#207016PurposeExpresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).DepositorInsertTIR1
UseTags9xMycExpressionYeastMutationPromoterpADH1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only