We narrowed to 1,482 results for: piggyBac
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Plasmid#167877PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2
Plasmid#175497PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.16_PB-Cx43_S368A-PGK-Blast
Plasmid#205561PurposeExpress human Cx43 with S368A mutationDepositorInsertCx43
UsePiggybacExpressionMammalianMutationS368APromoterEF1Available SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2
Plasmid#124176PurposepiggyBac-based Dox-inducible expression (moderate)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP4-PDGFR_EF1a_eBFP2
Plasmid#186306PurposePlasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertsAP4-PDGFR
eBFP2
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2
Plasmid#124175PurposepiggyBac-based Dox-inducible expression (very weak)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2
Plasmid#124174PurposepiggyBac-based Dox-inducible expression (weak)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH2B
Plasmid#133390PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneExpressionMammalianPromoterNo promoterAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg83-PDGFR_EF1a_iRFP670
Plasmid#186311PurposePlasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg83-PDGFR
iRFP670
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P9-PDGFR_EF1a_mPlum
Plasmid#186307PurposePlasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP9-PDGFR
mPlum
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP10-PDGFR_EF1a_mOrange
Plasmid#186308PurposePlasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line constructionDepositorInsertsAP10-PDGFR
mOrange
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P3-PDGFR_EF1a_iRFP670
Plasmid#186305PurposePlasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP3-PDGFR
iRFP670
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry
Plasmid#167902PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-CPspaH2B
Plasmid#133385PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG
Plasmid#175501PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
vas-Cas9.874Z
Plasmid#112687PurposeExpress hSpCas9 under vasa promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromotervasaAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only