165,528 results

1. pCap-SG5-Apr

   Plasmid

   #227579

   Purpose

   Capture plasmid with apramycin resistance and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-HIF2alpha-P405A/P531A-pcDNA3

   Plasmid

   #18956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-DIO-tdTomato

   Plasmid

   #99116

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   DIO-tdTomato
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM-CBE

   Plasmid

   #196293

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding CBE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. V5-BS2-NES (Cytosol)

   Plasmid

   #220341

   Purpose

   Mammalian expression vector BS2 in cytoplasm

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Tags

   NES (Nuclear exclusion sequence) and V5 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. LcV2-Hygro

   Plasmid

   #91977

   Purpose

   LentiCRISPR v2 with Hygro cloned in place of Puro

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. bdSUMO-HA2-GFP-HiBit

   Plasmid

   #171017

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-HA2-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. UbcH5c WT pET28a

   Plasmid

   #12643

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Expression

   Bacterial

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE:mCherry-p2a- bReaChES

   Plasmid

   #163037

   Purpose

   FLiCRE reporter gene for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   mCherry-p2a-bReaChES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0794

    Plasmid

    #142239

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF7 (ATF7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221_SLC7A9

    Plasmid

    #132245

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A9 (SLC7A9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pTXB1-AbaSFI

    Plasmid

    #86900

    Purpose

    Express CBD and intein tag containing AbaSI enzyme full length

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    AbaSDFI
    

    Tags

    CBD

    Promoter

    T7

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. psPAX2-D64V-NC-MS2

    Plasmid

    #122944

    Purpose

    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    NC-MCP (MS2 coat protein) fusion protein in Gag
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    D64V

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-GFP SNAP25

    Plasmid

    #170872

    Purpose

    Mammalian expression of His-GFP SNAP25.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    SNAP 25 (Snap25 Rat)
    

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1683

    Plasmid

    #142826

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TWIST1 (TWIST1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRVV210

    Plasmid

    #87628

    Purpose

    Drives Bxb1 expression in the D. melanogaster germ line.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1
    

    Expression

    Insect

    Promoter

    vasa

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBbB8k-csg-NbGFP

    Plasmid

    #166858

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCre-P2A-mCherry

    Plasmid

    #212105

    Purpose

    Expression of Cre and mCherry in mammalian cells

    Depositor

    Daekee Lee

    Insert

    Cre recombinase and mCherry
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJR014

    Plasmid

    #198813

    Purpose

    Natural light-gated anion channels. In vivo inhibition of neurons using blue light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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