159,530 results

1. mCarmine in pcDNA3

   Plasmid

   #109486

   Purpose

   mammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

   Depositor

   Oliver Griesbeck

   Article

   Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

   Insert

   mCarmine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH2.10_Gag-MCP-P2A-eGFP

   Plasmid

   #205525

   Purpose

   Express HIV Gag-MCP-P2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Gag-MCP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pME-Cas9

   Plasmid

   #63154

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC2A3

   Plasmid

   #132114

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A3 (SLC2A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDMyc-TNPO1

   Plasmid

   #86081

   Purpose

   A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Transportin-1 (TNPO1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQC-mCherryFP-GFP

   Plasmid

   #129102

   Purpose

   mCherryFP positive control

   Depositor

   Fangliang Zhang

   Article

   Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

   Insert

   mCherryFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA4-FLAG-Jhdm2a

   Plasmid

   #38136

   Depositor

   Toshinobu Nakamura, Toru Nakano

   Article

   Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

   Insert

   Jhdm2a (Kdm3a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   protein starts at aa115

   Promoter

   CMV

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. V48 ELP

   Plasmid

   #68395

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   V48 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VEGFR2-mCh

   Plasmid

   #108854

   Purpose

   Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

   Depositor

   Kalina Hristova

   Article

   Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Labeled with mCherry on the C-Terminus via a 3 am…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV

    Plasmid

    #24828

    Depositor

    Lin He

    Article

    Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTP284

    Plasmid

    #113312

    Purpose

    human Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDNA2.0 6H-TEV-KRAS

    Plasmid

    #111849

    Purpose

    Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCM66T: trimmed pCM66 backbone

    Plasmid

    #74738

    Purpose

    Small IncP-based broad host range replicating vector

    Depositor

    Mary Lidstrom

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG26

    Plasmid

    #35127

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    hph
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tdp43-EGFP construct4

    Plasmid

    #28197

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa216-414 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLX401-INK4A

    Plasmid

    #121919

    Purpose

    Doxycycline-inducible expression of p16-INK4A product of CDKN2A

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    p16-INK4A (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tet ON

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTriEx4-H6-FGAm (FlincG3)

    Plasmid

    #49202

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG3
    

    Use

    Tags

    His tag + other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Met to Lys in cpEGFP region similar to CGaMP3

    Promoter

    CMV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. piRFP

    Plasmid

    #31857

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Has Service

    Cloning Grade DNA

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dSpyCas9-p300-P2A-CASANOVA

    Plasmid

    #113129

    Purpose

    Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    dCas9-p300-P2A-CASANOVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge