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pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p5E-Runx1+23

Plasmid

#69602

Purpose

Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

Depositor

Owen Tamplin, Leonard Zon

Publication

Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

Inserts

Runx1 +23 intronic enhancer (Runx1 Mouse)
Mouse beta-globin minimal promoter

Use

5' gateway entry vector

Promoter

C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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anti-Notch1_E6-pBIOCAM5

Plasmid

#39344

Depositor

John McCafferty

Publication

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Human, Mouse)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Publication

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Expression

Bacterial

Promoter

T7

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMaCTag-P34

Plasmid

#120030

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJWV102-PL-dCas9

Plasmid

#85588

Purpose

Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

Depositor

Jan-Willem Veening

Publication

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

dCas9sp

Use

CRISPR

Expression

Bacterial

Promoter

PLsp

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-mTurquoise2-CNA35

Plasmid

#61602

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

Depositor

Maarten Merkx

Publication

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mTurqouise and CNA35

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWPXL-c-Myc

Plasmid

#36980

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

c-Myc (Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
FLX1.8MitoKaede

Plasmid

#28133

Depositor

Geoffrey Owens

Insert

MitoKaede

Use

Lentiviral

Tags

COX VIII signal sequence

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV5 Flag TGIF

Plasmid

#14047

Depositor

Joan Massague

Publication

Wotton et al J Biol Chem. 1999 Dec 24. 274(52):37105-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TGIF (TGIF1 Human)

Tags

Flag

Expression

Mammalian

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pTRIP-SFFV-mtagBFP-2A

Plasmid

#102585

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pp-mCAT1

Plasmid

#83948

Purpose

This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

Depositor

Vincent Lotteau, Philippe Mangeot

Publication

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

mCAT1 (Slc7a1 )

Expression

Mammalian

Promoter

CMV

Available since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO1

Plasmid

#24316

Depositor

Carl Novina

Publication

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO1 (AGO1 Human)

Tags

GST

Expression

Bacterial

Available since

April 7, 2010

Availability

Academic Institutions and Nonprofits only
pB-actin E6 E7

Plasmid

#13712

Depositor

Karl Munger

Publication

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HPV 16 E6 E7 (E6, E7 Human)

Expression

Mammalian

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pYOY152

Plasmid

#13987

Depositor

Linda Wordeman

Publication

Ovechkina et al J Cell Biol. 2002 Nov 25. 159(4):557-62.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLS-mP-Luc

Plasmid

#106253

Purpose

Luciferase lentivirus based enhancer assay vector

Depositor

Nadav Ahituv

Insert

Luciferase

Use

Lentiviral and Luciferase

Promoter

Derived from pGL4.23

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Publication

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHLmMBP-6

Plasmid

#72346

Purpose

Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Publication

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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myc-hAtg13

Plasmid

#31965

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Atg13 (ATG13 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles