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pLenti-PGK-RAP1A-E63-puro

Plasmid

#156167

Purpose

Lentiviral vector for expression of RAP1A-E63, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pmEos2-N1 GBP (GBP-mEos2)

Plasmid

#162876

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein tagged with mEos2

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.RING1B

Plasmid

#224712

Purpose

Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

RING1b (RNF2 Human)

Tags

6x histidine tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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A*02:02:01:01

Plasmid

#203277

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:02:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-lox-ERAS

Plasmid

#52417

Purpose

Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

ERAS (ERAS Human)

Use

Lentiviral

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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A*68:01:01

Plasmid

#203292

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*68:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-TEAD4-IDR

Plasmid

#166451

Purpose

Expresses fusion of mCherry and TEAD4-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR(89-230) (TEAD4 Human)

Expression

Mammalian

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-N-EP300

Plasmid

#166452

Purpose

Expresses fusion of mCherry and EP300-N-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, N-EP300(1-341) (EP300 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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eTd-CBEa

Plasmid

#196603

Purpose

Mammalian expression of eTd-CBEa

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*02:11:01

Plasmid

#203280

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:11:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*66:01:01

Plasmid

#203291

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*66:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-C-EP300

Plasmid

#166454

Purpose

Expresses fusion of mCherry and EP300-C-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, C-EP300(1795-2414) (EP300 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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sgAPC(B)

Plasmid

#85558

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-HA_N-human Bcl6

Plasmid

#137851

Purpose

Express Bcl6 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

B cell lymphoma 6 (BCL6 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pF CAG BFP2 IRES neo

Plasmid

#108175

Purpose

Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Blue fluorescent protein (BFP2)

Use

Lentiviral; Fluorescent protein

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PL-CRISPR.EFS.tRFP-gIfngr1-1

Plasmid

#166479

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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PL-CRISPR.EFS.tRFP-gIfngr1-2

Plasmid

#166480

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Prkd2 without kinase domain

Plasmid

#138411

Purpose

Express Prkd2 without kinase domain in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Prkd2 without kinase domain (PRKD2 Human)

Tags

HA

Expression

Mammalian

Mutation

kinase domain deleted from human Prkd2

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only