We narrowed to 26,705 results for: Cat

1. pJH4531

   Plasmid

   #132366

   Purpose

   Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-2 (daf-2 Nematode)
   

   Use

   Tags

   none

   Expression

   Worm

   Mutation

   Promoter

   Prgef-1

   Available since

   Sept. 22, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. L40C-CRISPR.EFS.dTomato

   Plasmid

   #89392

   Purpose

   Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH4

   Plasmid

   #52529

   Purpose

   serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   2x FLAG-tag
   

   Use

   Blunt cloning kit

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRG/III-hs-MBP-T43NTR-TrxA-H10

   Plasmid

   #121677

   Purpose

   Bacterial expression of neurotensin receptor fusion protein

   Depositor

   Reinhard Grisshammer, Richard Henderson

   Article

   Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

   Insert

   neurotensin receptor (Ntsr1 Rat)
   

   Use

   Tags

   TrxA-H10

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-42

   Promoter

   lac

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

5. px330 p300 gRNA

   Plasmid

   #165591

   Purpose

   Insertion of p300 degron

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   P300 (Smarca2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSilencer2.1-U6-T1-U6-AP3

   Plasmid

   #190828

   Purpose

   For mammalian expression of T1 and AP3, the RNA aptamers of ncOGT and GFP, respectively. Expression of each aptamer is driven by a U6 promoter.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Inserts

   T1 (an RNA aptamer of OGT)
   AP3 (an RNA aptamer of GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSC-SmBiT

   Plasmid

   #207632

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-SmBiT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-NPM NESD

   Plasmid

   #13283

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   NPM (NPM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of aa94-aa102

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

9. mEmerald-MCM3-N-22

   Plasmid

   #54166

   Purpose

   Localization: Nucleus, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

   Insert

   MCM3
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHiUGE V5-epitope donor ORF0

    Plasmid

    #200377

    Purpose

    Tagging endogenously expressed proteins with V5 epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    V5-epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30_Halotag_K73T_v2

    Plasmid

    #183692

    Purpose

    Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K73T in Halotag

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBB-II-I-GFP alpha isoform

    Plasmid

    #22146

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

14. GFP/WT SP M85

    Plasmid

    #89317

    Purpose

    Expression of mouse GFP/WT spastin M85 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2008 Feb 27;28(9):2147-57. d

    Insert

    SPAST M85 (Spast Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. c-myc promoter (4x TBE2 - wt)

    Plasmid

    #16599

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (4xTBE2 wt) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

16. FRB-polySH33-EGFP

    Plasmid

    #175792

    Purpose

    Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBG-TFII-I gamma

    Plasmid

    #22156

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pDEST-hPDI

    Plasmid

    #60555

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Use

    Tags

    His6-hPDI

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH3185

    Plasmid

    #132367

    Purpose

    Prgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-16a (daf-16 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Prgef-1

    Available since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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