We narrowed to 11,803 results for: nsf

1. pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199369

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOstar-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHC01

   Plasmid

   #106900

   Purpose

   Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

   Depositor

   Jonathan Silberg

   Article

   Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

   Inserts

   LasR
   Methyl Halide Transferase
   Ethylene Forming Enzyme
   Red Fluorescent Protein
   

   Expression

   Bacterial

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTarget TMEM97-P2A-mCardinal

   Plasmid

   #157747

   Purpose

   Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

   Depositor

   Andrew Kruse

   Article

   Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

   Insert

   TMEM97 (TMEM97 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pAAV-CAGGS-Flex/5'USS-GFP

   Plasmid

   #197884

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-ABE-NT-sgRNA

   Plasmid

   #112734

   Purpose

   AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

   Depositor

   Jin-Soo Kim

   Article

   Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

   Insert

   ABE-NT-sgRNA
   

   Use

   AAV

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV_pSyn_Mito-LOV-Turbo-V5

   Plasmid

   #199669

   Purpose

   expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   AAV

   Tags

   COX4I1 mito targeting sequence and V5

   Promoter

   Synapsin

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

   Plasmid

   #37087

   Purpose

   Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   NpHR3.0-EYFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   EYFP

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV_Actb NHEJ donor

   Plasmid

   #97316

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Str-STIM1-NN_ST-SBP-mCherry

   Plasmid

   #65263

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-cTNT-Cre-miR122 TS

    Plasmid

    #209784

    Purpose

    Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    miR122 ts
    

    Use

    AAV

    Promoter

    cTNT

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. qTAG-C-Solo-mScarlet-H2BC11

    Plasmid

    #207756

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

    Plasmid

    #176743

    Purpose

    Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Cx43-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-flex-GFP-shRNA-scramble

    Plasmid

    #182503

    Purpose

    Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
    

    Use

    AAV, Cre/Lox, and RNAi

    Expression

    Mammalian

    Promoter

    hSyn promoter

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_GWRev_Dest

    Plasmid

    #86954

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC84-pCR8-dCas9

    Plasmid

    #48218

    Purpose

    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A)
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-TGFα-GSY-SPM

    Plasmid

    #163615

    Purpose

    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    TGFa-GSY-SPM (TGFA Synthetic, Human)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

    Plasmid

    #129532

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIA4
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV_Actb MMEJ donor

    Plasmid

    #97315

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-SynI-CreOn-FlpOff-EGFP

    Plasmid

    #176274

    Purpose

    Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid

    #211777

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Spy-EZH2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge