We narrowed to 17,827 results for: JUN

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pMRXIP-GFP-CERT(PHD)(W33A)

Plasmid

#221761

Purpose

Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

CERT (CERT1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–116, mutated 33W to 33A

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZ (1079-1751)

Plasmid

#99341

Purpose

Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-548e-5p

Plasmid

#103634

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-548e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-548e-5p target (MIR548E Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7a-7

Plasmid

#56443

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab7a (RAB7A Human)

Tags

EGFP

Expression

Mammalian

Mutation

N117S in NM_004637.5

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-mTET1

Plasmid

#83569

Purpose

Expresses TET1 with a mutated catalytic domain in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

catalytic domain mutant H1672D, D1674A

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Kaede

Plasmid

#219433

Purpose

AAV expression vector for Kaede

Depositor

Yoshiho Ikeuchi

Article

Martins-Costa et al Cell Stem Cell. 2024 Jun 6;31(6):866-885

Insert

Kaede

Use

AAV

Promoter

CAG

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-i-pep

Plasmid

#47436

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-FL

Plasmid

#34597

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pHelper-T7IS1

Plasmid

#140631

Purpose

Expresses ShCAST under the T7 promoter and an empty sgRNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pIE-TGFB2-H6

Plasmid

#128500

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMG81-MBP-t-M.MpeI-N374K-His

Plasmid

#197985

Purpose

Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

Depositor

Rahul Kohli

Article

Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

Insert

MBP-t-M.MpeI-N374K-His

Tags

His and MBP

Expression

Bacterial

Mutation

M.MpeI-N374K

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-EGFP

Plasmid

#166143

Purpose

EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GAL1

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-mRuby3

Plasmid

#166144

Purpose

mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GAL1

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(36-235)

Plasmid

#170533

Purpose

Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTet01

Plasmid

#78283

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187950

Purpose

ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-DICER-Prom

Plasmid

#25851

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

Dicer promoter (DICER1 Human, gene ID 23405)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-SI-112

Plasmid

#131127

Purpose

C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Mutant EGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187948

Purpose

ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

ER50-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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