We narrowed to 11,712 results for: NSI

1. pLenti_EF1a_Flag_TP53_WT

   Plasmid

   #183112

   Purpose

   Expresses flag-tagged p53 in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2-UAS-zmiRFP

   Plasmid

   #108415

   Purpose

   Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zmiRFP
   

   Use

   Zebrafish

   Promoter

   UAS

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMal-Abl-PRM 5R

   Plasmid

   #112088

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   PRM-5R (ABL1 Human)
   

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

   Plasmid

   #210709

   Purpose

   This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCR4-Hsp68::lacZ-H11

   Plasmid

   #139099

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

   Depositor

   Len Pennacchio

   Article

   Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Hsp68

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCMV Puro DEST p38KTRmCerulean3

   Plasmid

   #59155

   Purpose

   Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVC-FUS-R521C

   Plasmid

   #175067

   Purpose

   expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   FUS glycine rich protein (FUS Human)
   

   Tags

   HA and mVenus + mCherry

   Expression

   Mammalian

   Mutation

   changed Arginin 521 to Cystein; mVenus: C-Termina…

   Promoter

   CMV Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

   Plasmid

   #232722

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI
   

   Expression

   Mammalian

   Mutation

   within evoAPOBEC1: H47E+S48A; within Cas9 D10A

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-idCas9-KRAB-Hygro donor

   Plasmid

   #199621

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

   Insert

   AAVS1-idCas9-KRAB-Hygro
   

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-GemininΔDbox-HA-Puro

    Plasmid

    #193770

    Purpose

    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    GemininΔDbox-HA (GMNN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Dbox removed (R23A, L26A). Codons switched at siR…

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2 TDP-43 5F-L YFP

    Plasmid

    #84914

    Purpose

    Mammalian expresion of TDP-43 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    F147L, F149L, F194L, F229L, F231L

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG_FBXL7

    Plasmid

    #171848

    Purpose

    pcDNA3-FLAG_FBXL7

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    F-box and leucine rich repeat protein 7 (FBXL7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-hygro-STING HAQ+V155M

    Plasmid

    #102601

    Purpose

    Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    V155M R71H-G230A-R293Q

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-3xHA-FnCas9

    Plasmid

    #130969

    Purpose

    3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    FnCas9
    

    Use

    CRISPR

    Tags

    3xHA-NLS and NLS-T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Archon1-KGC-EGFP-ER2-WPRE

    Plasmid

    #108423

    Purpose

    Archon1 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX304_zeo_mmIrf1

    Plasmid

    #160098

    Purpose

    Expresses murine Irf1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Irf1 (Irf1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-MCS-MED1long-IDR-YFP-NLS

    Plasmid

    #145276

    Purpose

    Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MED1-IDR long (MED1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR207 SARS-CoV-2 E

    Plasmid

    #141273

    Purpose

    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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