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pET-6xHis-GFP(-12)

Plasmid

#219420

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Publication

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-12)

Tags

MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA63_HelperΔgIII

Plasmid

#190433

Purpose

M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 genes I-V and VII-XI

Use

Synthetic Biology

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Plac-SO

Plasmid

#218358

Purpose

Empty vector with lacIq-Plac expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet1

Plasmid

#218360

Purpose

Empty backbone with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAS159

Plasmid

#194482

Purpose

synAR Cassette

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synAR Cassette

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS158

Plasmid

#194481

Purpose

synMR Part (Part 3)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synMR (Part 3)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS156

Plasmid

#194479

Purpose

synER Part (Part 3)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synER (Part 3)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pWS5390-CRISPRai-URA3

Plasmid

#182829

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJ2077.2

Plasmid

#140666

Purpose

Controller plasmid for TS circuit and cL and cR controls

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Controller plasmid for TS circuit and cL and cR controls

Use

Synthetic Biology

Expression

Bacterial

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Publication

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VP64

Plasmid

#79375

Purpose

Recruits VP64 to compatible gRNAs for transcriptional activation

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VP64

Use

CRISPR

Expression

Mammalian

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pYTK073

Plasmid

#65180

Purpose

Encodes ConRE' as a Type 5 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConRE'

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK079

Plasmid

#65186

Purpose

Encodes HygromycinR as a Type 6 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

HygromycinR

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR_TRE-->2xNLS_IFP_PGK_PuroR

Plasmid

#162238

Purpose

Lentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

NLS IFP

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Publication

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pZD-CJ1111

Plasmid

#175058

Purpose

rbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803

Depositor

David Nielsen

Publication

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHH03C_32

Plasmid

#120897

Purpose

Functional test of ECF sigma factors and their cognate promoters

Depositor

Domitilla Del Vecchio

Publication

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Insert

ECF32_1122

Use

Synthetic Biology

Expression

Bacterial

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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Tg(lyz:EGFP-Mmu.Rpl10a)

Plasmid

#128838

Purpose

RNA isolation from neutrophils via TRAP

Depositor

Anna Huttenlocher

Publication

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO2.1

Plasmid

#208017

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Publication

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Expression

Bacterial

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pQP-2460

Plasmid

#138973

Purpose

Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Publication

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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