We narrowed to 28,446 results for: REP

1. pCm C-term SMT3 NikJC199U

   Plasmid

   #174362

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Use

   Tags

   8xHis, SMT3, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCm N-term MBP NikJC199U

   Plasmid

   #174364

   Purpose

   Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Use

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS416-BXG-altTAG

   Plasmid

   #158146

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment (w/ TAG codon)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS416-BXG

   Plasmid

   #158145

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment (w/ TAG codon)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS416-BYG

   Plasmid

   #158144

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment (wild-type)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCTCON2-Aga1p-FAPB2.3.6

   Plasmid

   #158142

   Purpose

   Yeast display reporter for non-display yeast strains (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCTCON2-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158143

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   FAPB2.3.6L1TAG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJ414 - DANCER-1

   Plasmid

   #136385

   Purpose

   Expresses DANCER-1 dynamic protein

   Depositor

   Roberto Chica

   Article

   Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

   Insert

   DANCER-1
   

   Use

   Tags

   6x-His tag (N-term of insert)

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54I

   Promoter

   T7 promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-11a (GB1 WT - A34F/V39L)

   Plasmid

   #136610

   Purpose

   Expresses GB1 WT double mutant (A34F/V39L)

   Depositor

   Roberto Chica

   Article

   Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

   Insert

   GB1 WT double mutant (A34F/V39L)
   

   Use

   Tags

   6x-Histag

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: A34F - V39L

   Promoter

   T7 promoter

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SEPLuc-E2A-cyOFP1

    Plasmid

    #141084

    Purpose

    precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEPLuc-E2A-cyOFP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

    Plasmid

    #133343

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid

    #133345

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_blaA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LGhG-pHG165a

    Plasmid

    #90048

    Purpose

    Constitutive expression of a chimeric LacI:GalR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

    Insert

    LGhG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ150-lin26

    Plasmid

    #80357

    Purpose

    Pmex-5:GFP::H2B::PEST::lin-26 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::lin-26 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCFJ150-atg4.2

    Plasmid

    #80352

    Purpose

    Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCFJ150-set6

    Plasmid

    #80362

    Purpose

    Pmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::set-6 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCFJ150-ets4

    Plasmid

    #80355

    Purpose

    Pmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::ets-4 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ150-mex3

    Plasmid

    #80361

    Purpose

    Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::mex-3 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ150-mbk2

    Plasmid

    #80360

    Purpose

    Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-CAG-zNanogHD-pgk-hph

    Plasmid

    #74910

    Purpose

    Mammalian expression of zNanog Homeodomain

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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