We narrowed to 11,803 results for: nsf

1. pAAV-iCre-2A-venus

   Plasmid

   #182499

   Purpose

   Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   iCre-2A-Venus
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-eYFP-3x-miR204-5p-TS

   Plasmid

   #117380

   Purpose

   An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEA02

   Plasmid

   #172193

   Purpose

   Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.

   Depositor

   Jean-Luc Pernodet

   Article

   Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

   Insert

   attL
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA-T-intron(Neo)-G(LNL)

   Plasmid

   #36908

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (i.e., ATG, start codon as a first exon)
   GFP
   beta-globin intron
   Neo
   

   Expression

   Mammalian

   Mutation

   deleted nucleotides before nucleotide 275 and ins…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)

   Plasmid

   #113762

   Purpose

   AAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

   Depositor

   Luke Lavis

   Article

   Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

   Insert

   tdNfsB(F124W)-mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F124W

   Promoter

   CAG

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xG1 PylT sfGFP 150TAG

   Plasmid

   #154767

   Purpose

   plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

   Plasmid

   #114378

   Purpose

   expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-KA2N-EGFP (NEWENTRY )
   

   Use

   AAV

   Tags

   EGFP and KA2N

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

   Plasmid

   #114101

   Purpose

   fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

   Insert

   Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPAT-2XStrep

   Plasmid

   #125620

   Purpose

   expresses human PPAT protein with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   PPAT (PPAT Human)
   

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM-AAV-mSncg-0.66kb-EGFP

    Plasmid

    #153165

    Purpose

    Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-synp-F-H2B-GCaMP6f

    Plasmid

    #102994

    Purpose

    Expresses histone fused GCaMP6f under the synapsin promoter

    Depositor

    David Cox

    Article

    Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

    Insert

    H2B-GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human synapsin I promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hRREB1-RFP

    Plasmid

    #22924

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

    Plasmid

    #114376

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP, Kv2.1C, and TlcnC

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCsy3-VPR-Csy4

    Plasmid

    #153943

    Purpose

    Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cells

    Depositor

    Zhou Songyang

    Article

    Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

    Insert

    Csy3-VPR, Csy4
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCsy1-Csy2

    Plasmid

    #153942

    Purpose

    Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cells

    Depositor

    Zhou Songyang

    Article

    Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

    Insert

    Csy1, Csy2
    

    Use

    AAV and CRISPR

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

    Plasmid

    #158989

    Purpose

    Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-SID4X
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

    Plasmid

    #109318

    Purpose

    Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    SaCas9 RuvC gRNA
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEP306-pAAV-EFS-MCS3-pA

    Plasmid

    #113682

    Purpose

    EFS driven Multi Cloning Site-3.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_4xMx PylT C41CA_GFP 150TAG

    Plasmid

    #140017

    Purpose

    Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. aav-SYN-FLEX-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113763

    Purpose

    AAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    Synapsin

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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