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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Actin-Vhh-Halo-Tag

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

Plasmid

#175524

Purpose

Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7 and Lyn11-SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

Plasmid

#215002

Purpose

Shuttling vector for an ERphagy reporter

Depositor

Wade Harper

Insert

KDEL

Use

Gateway

Tags

HaloTag(7)-mGFP

Expression

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

Plasmid

#215014

Purpose

Express an autophagy (LC3) reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

MAP1LC3B (Map1lc3b Rat)

Use

Lentiviral

Tags

HaloTag(7)-mGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 122-130 nsp1

Plasmid

#175425

Purpose

Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 122-130 nsp1

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 118-180 nsp1

Plasmid

#175424

Purpose

Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 118-180 nsp1

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminus of nsp1

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7 (zebrafish) C49S and C117S

Plasmid

#108913

Purpose

Express C49S/C117S double mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7

Use

Tags

Halo-TEV

Expression

Mammalian

Mutation

Cysteine 49 to Serine, Cysteine 117 to Serine

Promoter

SP6

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)

Plasmid

#216717

Purpose

Expresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-RGD-PILATeS

Use

Tags

6xHis and HaloTag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)

Plasmid

#216715

Purpose

Expresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-RGD-PILATeS

Use

Tags

6xHis and HaloTag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

Plasmid

#215011

Purpose

Express a Mitophagy reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

pSu9 (Presequence of Fo-ATPase subunit 9)

Use

Lentiviral

Tags

HaloTag(7)-mGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

Plasmid

#175427

Purpose

Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E55A E57A K58A

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E55A E57A K58A

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

Plasmid

#62458

Purpose

expresses his-tagged C151S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Use

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 151 of Keap1 to serine

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Plasmid

#226514

Purpose

Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

Depositor

Kai Johnsson

Article

Kühn et al bioRxiv 2024.08.28.610127

Insert

HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

Plasmid

#159696

Purpose

E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)

Use

Tags

His6-Halotag-tev

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

Plasmid

#182012

Purpose

CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR-HaloTag7-P30-SNAP-tag-KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

Plasmid

#199457

Purpose

Expression of dCas13-VPR in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

dCas13

Use

Lentiviral

Tags

VPR-T2A-HaloTag

Expression

Mammalian

Mutation

Nuclease-dead H133A and H1058A

Promoter

CAG

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

Plasmid

#62459

Purpose

expresses his-tagged C288S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Use

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 288 of Keap1 to serine

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159872

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag

Plasmid

#185683

Purpose

Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

(WLLI-GSGS)[359-362]

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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