We narrowed to 2,905 results for: BFP

1. lenti-gRNA

   Plasmid

   #226867

   Purpose

   Lentiviral expression of Sp-gRNA with BlpI and BstXI restriction sites for gRNA spacer cloning. Also expresses BFP.

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Lenti Sp-gRNA
   

   Use

   Lentiviral

   Promoter

   mU6

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBW842_pCAG-pExpr-FP-DECODER

   Plasmid

   #87551

   Purpose

   Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mtagBFP, EGFP, iRFP720, mRuby2
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

   Plasmid

   #68405

   Purpose

   The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
   FlpO
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Promoter

   Synthetic Probasin ARRx2 and U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRMTK-clo41

   Plasmid

   #204016

   Purpose

   Cloning plasmid containing the BFP insert with a chloramphenicol resistance marker

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Blue fluorescent protein
   

   Expression

   Plant

   Promoter

   N/A

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. InterTag

   Plasmid

   #186910

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   SpyTag003-sfCherry11-PDGFR
   

   Expression

   Mammalian

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. InterTag-3xL-Δtag

   Plasmid

   #186914

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   sfCherry11-(GGGGS)3-PDGFR
   

   Expression

   Mammalian

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. IDP378

   Plasmid

   #87005

   Purpose

   plasmid for cell culture expression of SYNseq components

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Insert

   preLib-bfp-4xboxB
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC026

   Plasmid

   #227598

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMN-013

   Plasmid

   #195417

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF5

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. InterTag-3xL

    Plasmid

    #186913

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene

    Depositor

    Allen Liu, Taekjip Ha

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    SpyTag003-sfCherry11-(GGGGS)3-PDGFR
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC-U63-QtgRNA2.1-BR

    Plasmid

    #170513

    Purpose

    gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC025

    Plasmid

    #227597

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMN-009

    Plasmid

    #195413

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF1

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLG1-puro Non-targeting sgRNA 3

    Plasmid

    #109003

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMN-018

    Plasmid

    #195422

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF10

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMN-011

    Plasmid

    #195415

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF3

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF3 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMN-010

    Plasmid

    #195414

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF2

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF2 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMN-012

    Plasmid

    #195416

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF4

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF4 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMN-014

    Plasmid

    #195418

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF6

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF6 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMN-015

    Plasmid

    #195419

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF7

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF7 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMN-016

    Plasmid

    #195420

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF8

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF8 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMN-017

    Plasmid

    #195421

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF9

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF9 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMN-019

    Plasmid

    #195423

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF11

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF11 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCVL SFFV Sbf-Sal linker

    Plasmid

    #50434

    Purpose

    Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6Rosa-CAG-Gal4-Rad18UBD

    Plasmid

    #119012

    Purpose

    Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. InterTag-2xL

    Plasmid

    #186912

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance gene

    Depositor

    Allen Liu, Taekjip Ha

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    SpyTag003-sfCherry11-(GGGGS)2-PDGFR
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. InterTag-1xL

    Plasmid

    #186911

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

    Depositor

    Allen Liu, Taekjip Ha

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    SpyTag003-sfCherry11-(GGGGS)1-PDGFR
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-Gal4-RNF169UBD

    Plasmid

    #119013

    Purpose

    Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-BRCA1

    Plasmid

    #119007

    Purpose

    Mammalian expression of human BRCA1 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. sg2.02 16x(MS2) MUC4.1

    Plasmid

    #101154

    Purpose

    gRNA with 16 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TetO-FUW-VdC9BV

    Plasmid

    #62195

    Purpose

    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

    Depositor

    Kam Leong

    Article

    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

    Insert

    VP64dCas9BFPVP64
    

    Use

    Lentiviral

    Tags

    Two VP64s tagged to dCas9 fused to BFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive) Cas9 (dCas9)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. sg14x(MS2) MUC4.1

    Plasmid

    #101153

    Purpose

    gRNA with 14 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Dox-Akt

    Plasmid

    #207328

    Purpose

    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

    Depositor

    Jared Toettcher

    Article

    Underhill et al Development. 2023 Aug 15;150(16):dev2016

    Insert

    rac protein kinase-alpha (AKT1 Human)
    

    Tags

    myristoylation tag - BFP

    Expression

    Bacterial

    Mutation

    range: 129 - 480

    Promoter

    Tet Response Element

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. sg2.02 MUC4.1

    Plasmid

    #101152

    Purpose

    gRNA with two MCP binding sites

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    mU6

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMK1224

    Plasmid

    #84221

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge