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Showing: 1681 - 1720 of 1734 results

  1. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid
    #167573
    Purpose
    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    Ribo-GCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167573-plasmid-map-sequence-id-334459

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  2. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

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  4. pRSET-iGluSnFR4f

    Plasmid
    #234444
    Purpose
    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f
    Use
    Tags
    6xHis-Xpress epi
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234444-plasmid-map-sequence-id-464950

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  5. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid
    #225949
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    C100A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225949-plasmid-map-sequence-id-446851

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  6. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid
    #225950
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Additional cysteine inserted after Cys-100
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225950-plasmid-map-sequence-id-446852

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  7. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid
    #225955
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Truncated CKAP4 (1-131) lacking the luminal domain
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225955-plasmid-map-sequence-id-446861

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  8. pLV-SFFV-RTN4-WPRE-UbC-Emerald

    Plasmid
    #225938
    Purpose
    Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    RTN4 (RTN4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225938-plasmid-map-sequence-id-446791

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  9. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

    Plasmid
    #225940
    Purpose
    Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    STIM2 (STIM2 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225940-plasmid-map-sequence-id-455851

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  10. pCHMP4B-NS3-Blue

    Plasmid
    #223534
    Purpose
    Mammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP4B (CHMP4B Human)
    Use
    Tags
    NS3 Hepatitis C protease and mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223534-plasmid-map-sequence-id-443365

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  13. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid
    #225947
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S3E S17E S19E
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225947-plasmid-map-sequence-id-446849

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  14. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid
    #225948
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S3A S17A S19A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225948-plasmid-map-sequence-id-446850

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  17. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

    Plasmid
    #225956
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Full-length CKAP4 with additional luminal domain …
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225956-plasmid-map-sequence-id-446863

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  18. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid
    #225939
    Purpose
    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    STIM1 (STIM1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225939-plasmid-map-sequence-id-446835

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  19. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid
    #225944
    Purpose
    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    ATL1 (ATL1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225944-plasmid-map-sequence-id-446838

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  20. pAAV-syn-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234438
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234438-plasmid-map-sequence-id-465745

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  21. pAAV-syn-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234437
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234437-plasmid-map-sequence-id-465724

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  22. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234452
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234452-plasmid-map-sequence-id-465722

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  23. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234451
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234451-plasmid-map-sequence-id-465732

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  24. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234436
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234436-plasmid-map-sequence-id-465719

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  26. pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234445
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    GFAP
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234445-plasmid-map-sequence-id-465739

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  27. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234446
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    GFAP
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234446-plasmid-map-sequence-id-465727

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  28. pAAV-syn-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234435
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234435-plasmid-map-sequence-id-465726

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  30. pAAV-CAG-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234450
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234450-plasmid-map-sequence-id-465729

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  35. pAAV-CAG-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234449
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234449-plasmid-map-sequence-id-465741

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  36. pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234447
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234447-plasmid-map-sequence-id-465730

    Enlarge

  37. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234441
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234441-plasmid-map-sequence-id-465749

    Enlarge

  38. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234442
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234442-plasmid-map-sequence-id-465693

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  39. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid
    #203797
    Purpose
    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Article
    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d
    Inserts
    PIK3CA (PIK3CA Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203797-plasmid-map-sequence-id-401572

    Enlarge

  40. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

    Enlarge

Showing: 1681 - 1720 of 1734 results