We narrowed to 987 results for: Streptococcus

1. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRM053 His-TEV-Cas9 triNLS 0C

   Plasmid

   #196244

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRM049 His-TEV-Cas9 triNLS 2C

   Plasmid

   #196245

   Purpose

   Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EFS-SpdCas9-Dnmt3A/3L-WT

   Plasmid

   #222497

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

   Plasmid

   #132667

   Purpose

   Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-ODD-T2A-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Promoter

   4xHRE_YB TATA

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE3GV-SpdCas9-Dnmt3A/3L-WT

   Plasmid

   #222502

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   TRE3GV and hPGK

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

   Plasmid

   #136652

   Purpose

   Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Cas9-T2A-STOP-TdT

   Plasmid

   #126425

   Purpose

   Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Mutation

   alanine-6 of TdT has been changed to a STOP codon

   Promoter

   pCMV

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY70

   Plasmid

   #130948

   Purpose

   A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dxcas9 (3.7)
   tetR
   rhaS
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9 for dCas9; Sy…

   Promoter

   Pcon, PpspA-LEA3B3, PrhaB, and Ptet

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY164

    Plasmid

    #184140

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R3, PrhaB, and Ptet

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY61

    Plasmid

    #130928

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid

    #136659

    Purpose

    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid

    #222498

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222499

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY162

    Plasmid

    #184138

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R1, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY163

    Plasmid

    #184141

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R2, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY168

    Plasmid

    #184142

    Purpose

    Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-Ar2, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid

    #136657

    Purpose

    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid

    #136654

    Purpose

    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF1a-PEmax-P2A-GFP

    Plasmid

    #202000

    Purpose

    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

    Depositor

    Daniel Geschwind

    Insert

    PEmax-P2A-GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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