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pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-TGFB1

Plasmid

#164427

Purpose

AAV plasmid expressing TGFB1 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb1 (Tgfb1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTE4254

Plasmid

#89050

Purpose

Expresses St chimeric gRNA and human codon-optimized StCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

St chimeric gRNA
hStCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRJPaph-sYFP2

Plasmid

#67033

Purpose

Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
sYFP2

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mChe

Plasmid

#67034

Purpose

Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mCherry

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 GOF

Plasmid

#53200

Purpose

Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

R67S/D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pHTN HaloTag CMV-neo/ERGIC2-variant

Plasmid

#51477

Purpose

Expresses ERGIC2-variant in mammalian cells, HaloTag for cellular localization

Depositor

Simon Kwok

Article

Kwok et al DNA Cell Biol. 2013 Dec 4.

Insert

ERGIC2 (ERGIC2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

4 base deletion at codon #189

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
aq-CFP

Plasmid

#36073

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein subunit alpha q (Gnaq Mouse)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between amino acid 124 and 125

Promoter

CMV

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pECE-HA-AMPKalpha2 M93G

Plasmid

#31655

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
eGFP-SUN1

Plasmid

#213515

Purpose

Expresses human eGFP-SUN1 in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319

Insert

SUN1 (SUN1 Human)

Expression

Mammalian

Mutation

SUN1 has a silent mutation on its gene (nucleotid…

Promoter

CMV

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

Plasmid

#198515

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP

Plasmid

#198389

Purpose

GCN1 R2312A expresson

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

R2312A mutation in RWDBD (RWD binding domain)

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-GATA4

Plasmid

#192896

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

GATA4 (GATA4 Human)

Tags

HA

Expression

Mammalian

Mutation

Codon-optimized

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNR4A1-donor

Plasmid

#112295

Purpose

CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP

Depositor

Kevin White

Insert

NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)

Use

CRISPR

Mutation

hg38:12:52058840:C>T, hg38:12:52058930:A>C,…

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-mTET1_CD

Plasmid

#83571

Purpose

Expresses a mutant catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted, catalytic domain…

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (1-479) 3x FLAG

Plasmid

#74262

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-479

Promoter

5’LTR

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only