We narrowed to 10,525 results for: SEC

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CMV-LUC2CP/intron/ARE

Plasmid

#62858

Purpose

splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

Depositor

Gideon Dreyfuss

Article

Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

insertion of chimeric intron at nueotide poclsiti…

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF

Plasmid

#74924

Purpose

Mammalian expression of human NSF full-length (744 aa)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1

Plasmid

#22027

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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ABE7.10-F148A

Plasmid

#132946

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

ABE7.10(F148A)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

ABE7.10(F148A)

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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PD2529 human beta1 full

Plasmid

#221401

Purpose

Mammalian expression of human integrin beta1 full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162383

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pD2sE_SC14

Plasmid

#219717

Purpose

DV2 stable E dimer

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC14

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

A259W, T262R, F279W, T280P

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pP{ELAV-GeneSwitch}

Plasmid

#83957

Purpose

Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

Depositor

Haig Keshishian

Article

Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

Insert

fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Human, Budding Yeast)

Expression

Insect

Promoter

elav

Available Since

Dec. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR3DX1-COMP5AP-AviTag-9xHis

Plasmid

#157338

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DX1 (KIR3DX1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pACgp67B-Her2

Plasmid

#10794

Depositor

Judy Lieberman

Article

Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

Insert

ErbB2 ScFv (ERBB2 Human)

Tags

6His

Expression

Insect

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10FLAG

Plasmid

#157736

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

His10 + 3xFLAG, enterokinase-cleavable

Expression

Insect

Promoter

polyhedrin

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTEF:ATP

Plasmid

#92179

Purpose

HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)

Use

Integration and expression into yeast.

Tags

Yeast codon optimized mseCFP (donor) and cp173-mV…

Expression

Yeast

Promoter

TEF1p

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIghg2b

Plasmid

#127155

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Ighg2b (Ighg2b Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEntr_Myc-BirA-G3-ER

Plasmid

#173814

Purpose

Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Depositor

Norbert Perrimon

Article

Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

Insert

Myc-BirA-G3-ER

Use

Gateway shuttle vector

Tags

Myc

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mMD2-proA

Plasmid

#187878

Purpose

baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

MD2 (Ly96 Mouse)

Expression

Insect

Mutation

includes residues 19–160 of mouse MD-2

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-PA-Rac1

Plasmid

#22024

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP

Plasmid

#221891

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

TAF15 (TAF15 Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBac1-BacMam-4x-PyOtR

Plasmid

#217364

Purpose

Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

4 copies of pyrrolysyl-tRNA mutant PyOtR

Use

Baculoviral

Expression

Insect and Mammalian

Promoter

U6

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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sc-WDR24-SEH1L

Plasmid

#237418

Purpose

Transient expression of human WDR24-SEH1L "single chain" in the GATOR2 complex

Depositor

David Sabatini

Article

Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

Insert

WDR24-SEH1L fusion (WDR24, SEH1L Human)

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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