We narrowed to 26,138 results for: Vit

1. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

   Plasmid

   #205463

   Purpose

   A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-A; UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

   Plasmid

   #205465

   Purpose

   A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-C; UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. non-targeting control gRNA (BRDN0001147780)

   Plasmid

   #80226

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE4560

   Plasmid

   #107526

   Purpose

   Expresses As crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and human U6

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p260 pCMV-CRE-M-AR(wt)

   Plasmid

   #12494

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

   Insert

   CRE-M AR (AR Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   CRE-M and AR ligand binding domain

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

8. WEE1 gRNA (BRDN0001145570)

   Plasmid

   #77368

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human WEE1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   WEE1 (WEE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

   Plasmid

   #63060

   Purpose

   Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   nls-mRuby2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

    Plasmid

    #187994

    Purpose

    Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    mucin2 D1D2D'D3 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGEX-6P1-hNF2-AR mutant

    Plasmid

    #107149

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A585W-R588K

    Promoter

    Ptac

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. ROCK1 gRNA (BRDN0001147709)

    Plasmid

    #77593

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pLKO.1P POLD1_1

    Plasmid

    #160791

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P REV3L_2

    Plasmid

    #160798

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1P REV3L_1

    Plasmid

    #160797

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1P POLA1_2

    Plasmid

    #160790

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1P POLA1_1

    Plasmid

    #160789

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRetroX-TRE3G_NT-GSDMD_I105N_

    Plasmid

    #201174

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only, I105N

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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