We narrowed to 29,897 results for: des.2

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pB-actin 6bE6

Plasmid

#37435

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 6bE6 (E6 HPV)

Expression

Mammalian

Promoter

Human B-actin

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
pHCMV-EcoEnv

Plasmid

#15802

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

MLV env (ecotropic)

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pBS598 EF1alpha-EGFPcre

Plasmid

#11923

Purpose

Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pUbiGUSPlus

Plasmid

#64402

Purpose

Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.

Depositor

Claudia Vickers

Article

Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E

Insert

ubiquitin (Ubi-1) promoter

Tags

GUSPlus reporter

Expression

Plant

Promoter

Ubi-1

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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SIRT4 Flag

Plasmid

#13815

Purpose

Mammalian expression of human SIRT4 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT4 (SIRT4 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pJFRC19-13XLexAop2-IVS-myr::GFP

Plasmid

#26224

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

myr::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pLOX-gfp-iresTK

Plasmid

#12243

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

GFP, HSV1-TK

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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SIRT6 Flag

Plasmid

#13817

Purpose

Mammalian expression of human SIRT6 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT6 (SIRT6 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pN7-S15

Plasmid

#190665

Purpose

Expresses N7 ribosomal protein S15

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7 S15

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SIRT5 Flag

Plasmid

#13816

Purpose

Mammalian expression of human SIRT5 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT5 (SIRT5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pJTM-T7-mNeongreen-6xHis

Plasmid

#210868

Purpose

Plasmid used for expressing his--tagged mNeongreen for protein purification.

Depositor

Richard Murray

Article

Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

Insert

mNeongreen-6xHistag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC18-8XLexAop2-mCD8::GFP

Plasmid

#26225

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pJFRC7-20XUAS-IVS-mCD8::GFP

Plasmid

#26220

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pET303_sumocodo_GFP_codoopti

Plasmid

#210393

Purpose

Express SUMO-GFP in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

GFP

Tags

SUMO-tag

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GW1CMV-Perceval

Plasmid

#21737

Purpose

can be used to monitor the ATP:ADP ratio during live-cell imaging

Depositor

Gary Yellen

Article

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

5'UTR

Expression

Mammalian

Mutation

improved Kozak sequence

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-HA3-STONIN1

Plasmid

#177580

Purpose

Expresses Stonin1 in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

Insert

STONIN1

Tags

3XHA and GFP

Expression

Mammalian

Mutation

V334A- please see depositor comments

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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nls-NgAgo-GK

Plasmid

#78253

Purpose

Mammalian expression of NgAgo (Argonaute from N. gregoryi).

Depositor

Chunyu Han

Article

Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

NgAgo

Tags

a nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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