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pNX3b-PK6

Plasmid

#78362

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

6xPK
hygMX6

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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LI D-E HA

Plasmid

#54449

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C HA

Plasmid

#54448

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pR184

Plasmid

#47952

Purpose

rtTA positive feedback loop with three tet operators in the GAL1 promoter

Depositor

Attila Becskei

Publication

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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SK-YFP-ST1-A

Plasmid

#48665

Purpose

Bacterial ST1 repression YFP reporter: protospacer A

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

ST1 prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pPD95.79-wVenus

Plasmid

#37466

Depositor

Hitoshi Sawa

Publication

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH35C-epegRNA-ePPEplus

Plasmid

#246795

Purpose

Express ePPEplus and pegRNA in plant cells (protoplast or callus) for prime editing

Depositor

Caixia Gao

Publication

Li et al Nat Biotechnol. 2026 Jun 5. doi: 10.1038

Insert

pegRNA, ePPEplus, HygR

Use

CRISPR

Available since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TurboID-TM

Plasmid

#231498

Purpose

Expressing TurboID on the cell surface of mammalian cells

Depositor

Baljit Khakh

Publication

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

TurboID

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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ULK1 KO sgRNA

Plasmid

#207561

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CCCGCCTGCGCCATGGAGCC

Expression

Mammalian

Promoter

CMV and U6

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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ATG16L1 sgRNA

Plasmid

#207563

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

GCAGCAAGTGACATGTCGTC

Expression

Mammalian

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Publication

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMZ-314

Plasmid

#195459

Purpose

Lentiviral Integratable, ABA-inducible expression of synZiFTR: ZF1-p65

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1

Use

Lentiviral

Expression

Mammalian

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Publication

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Expression

Bacterial

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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hiSIIN

Plasmid

#185662

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Publication

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Publication

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLN592 (FuGW-S(USF1)p-mKate2)

Plasmid

#105204

Purpose

synthetic promoter USF1 drives mKate2 expression

Depositor

Timothy Lu

Publication

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfvAChT-1.8k-Venus-WPRE

Plasmid

#156389

Purpose

Expresses Venus under vAChT gene promoter

Depositor

Shuji Kaneko

Publication

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-PuroTK-L2

Plasmid

#141005

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Expression

Mammalian

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMpGWB403-Lifeact-Citrine

Plasmid

#100582

Purpose

Binary vector for expression of Citrine-fused Lifeact (Lifeact-Citrine) to visualize actin filaments in plants

Depositor

Yutaka Kodama

Publication

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Lifeact-Citrine

Expression

Plant

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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