We narrowed to 11,271 results for: 158
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Plasmid#188263PurposePlasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnERb446 (ESR1 Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgSNRK
Plasmid#138694PurposeExpresses a human SNRK-targeting sgRNA and Cas9DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-LRRK2 WD40
Plasmid#139211PurposeHuman LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
A*33:01:01
Plasmid#203288PurposeOverexpress HLA alleleDepositorInsertA*33:01:01 (HLA-A Human)
ExpressionMammalianAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-Gluc-RMA-IRES-EGFP
Plasmid#189628PurposeExpresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianPromoterIRES and TREAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1472
Plasmid#141825PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-D672G-GFP
Plasmid#78919PurposeMammalian expression of BAP1 with D672G and an EGFP fusionDepositorAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
Plasmid#186968PurposeLentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.DepositorInsertpEF-H2B-mCherry-T2A-rTetR-Dest-SV40
UseLentiviralTagsrTetR fusionExpressionMammalianPromoterEF-1alpha promoterAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-A95D-GFP
Plasmid#78914PurposeMammalian expression of BAP1 with A95D and an EGFP fusionDepositorAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNT.3#3/Cre
Plasmid#173662PurposeExpresses a NT.3-targeting gRNA and Cre-recombinaseDepositorInsertNon-targeting gRNA
UseCRISPR, Cre/Lox, and LentiviralAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29(+exo) (LM2759)
Plasmid#208964PurposeUnfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-S63C-GFP
Plasmid#78911PurposeMammalian expression of BAP1 with S63E and an EGFP fusionDepositorAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
A*03:02:01
Plasmid#203294PurposeOverexpress HLA alleleDepositorInsertA*03:02:01 (HLA-A Human)
ExpressionMammalianAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-F81V-GFP
Plasmid#78912PurposeMammalian expression of BAP1 with F81V and an EGFP fusionDepositorAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
nLuc_AP10_PPVs_P9mS
Plasmid#119302PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logicDepositorInsertsplit nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#196545PurposeDonor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine reporter
UseSynthetic BiologyExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYL236
Plasmid#173184PurposeCRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDK2-3XFlag-pBabe
Plasmid#73557Purposevector encoding a fusion protein between CDK2 and 3 Flag tagsDepositorAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1471
Plasmid#143814PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0747 (pDonor_L-MmeI)
Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonExpressionBacterialMutationTGTTGATG --> TGTTGGAGAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only