We narrowed to 9,904 results for: UTY

1. BII-ChiNW-IGF2

   Plasmid

   #133383

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   IGF2 (IGF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

   Plasmid

   #197066

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   CRISPR

   Tags

   FKBP and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn1-DIO-VGLUT2:miniSOG

   Plasmid

   #214200

   Purpose

   For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

   Depositor

   Thomas Hnasko

   Article

   Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

   Insert

   Slc17a6 tagged with miniSOG and hemagglutinin
   

   Use

   AAV and Cre/Lox

   Tags

   miniSOG (tagged with HA) is fused to the first in…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEND-304_Cacnes_tetR

   Plasmid

   #225619

   Purpose

   C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   TetR
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEND-375_Cacnes_phlF

   Plasmid

   #225620

   Purpose

   C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   PhlF
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CHRM1-DuET

   Plasmid

   #213207

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CHRM1 (CHRM1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-myc-HLA-B57

   Plasmid

   #135516

   Purpose

   Mammalian expression of myc-tagged HLA-B*57:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*57:1 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATG-1929

   Plasmid

   #108712

   Purpose

   CBR2opt in pF4Ag expression vector with T7 and CMV promoter

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   T7, CMV

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSW003-PpsbA-mNeonGreen

   Plasmid

   #205019

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   PpsbA

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-HLA-B15

    Plasmid

    #135513

    Purpose

    Mammalian expression of myc-tagged HLA-B*15:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*15:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. nLYN267-gs10-NLS-VPR

    Plasmid

    #188265

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nLYN267 (LYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-human CSF-1R-Y969F,S301-IRES—GFP

    Plasmid

    #115524

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    human CSF-1 receptor constitutively activated mutant (CSF1R Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Y969F,S301

    Promoter

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDule2-3-nitroTyrosine (5B)

    Plasmid

    #85499

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. RXFP1-DuET

    Plasmid

    #213373

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    RXFP1 (RXFP1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TA1-DuET

    Plasmid

    #213382

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    TA1 (TAAR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1 puro shSLC2A1-1

    Plasmid

    #193702

    Purpose

    Constitutive lentiviral expression of SLC2A1 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    SLC2A1 (SLC2A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-PdTK-CAG-mCh-[uBglII]

    Plasmid

    #107280

    Purpose

    puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FPR3-DuET

    Plasmid

    #213241

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    FPR3 (FPR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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