We narrowed to 15,270 results for: grn

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PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

Plasmid

#154002

Purpose

Empty SgRNA expression system matching CasRx

Depositor

Hui Yang

Publication

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SgRNA expression system

Use

CRISPR

Promoter

U6, EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pUS2-SH231

Plasmid

#115150

Purpose

Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

Depositor

Raymond Monnat, Michael Phelps

Publication

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dual SH231 gRNAs

Use

CRISPR

Tags

3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

Expression

Mammalian

Promoter

U6, CBh

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP002

Plasmid

#103872

Purpose

Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

Depositor

Jean-Marc Daran

Publication

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Inserts

hph
Spcas9 D147Y P411T

Use

CRISPR

Tags

NLS

Expression

Yeast

Mutation

D147Y P411T

Promoter

Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-mTubb3

Plasmid

#87116

Purpose

AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA

Use

AAV and CRISPR

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LsgRNA-MS2

Plasmid

#235597

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

Depositor

Ting Zhou

Publication

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Publication

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcU6_1 sgRNA

Plasmid

#92395

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.1 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.1

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJR100

Plasmid

#187240

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORANGE Gria1-GFP KI

Plasmid

#131489

Purpose

Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDY1623 NOLC1 sgRNA 2

Plasmid

#234834

Purpose

sgRNA 2 for NOLC1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NOLC1 sgRNA (NOLC1 Human)

Use

CRISPR

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-GEMSgRNA2

Plasmid

#204768

Purpose

pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Depositor

Kate Meyer

Publication

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

guide RNA targeting GEMS m6A sensor mRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-B

Plasmid

#177787

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSCAR_sgRNA_puro-mKate-lox5171

Plasmid

#162077

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Publication

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AXL gRNA (BRDN0001146797)

Plasmid

#76700

Purpose

3rd generation lentiviral gRNA plasmid targeting human AXL

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AXL (AXL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0005

Plasmid

#42245

Purpose

gRNA targeted to zebrafish gene gsk3b

Depositor

Keith Joung

Publication

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-gsk3b (gsk3b Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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