20,112 results

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pBUN411

Plasmid

#50581

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

3×FLAG-NLS-zCas9-NLS
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Mutation

maize codon optimized

Promoter

OsU3p and Ubi1p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5-25BoxB

Plasmid

#60817

Purpose

Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP

Depositor

Maria Carmo-Fonseca

Article

Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:

Insert

25x BoxB

Use

Synthetic Biology

Expression

Mammalian

Mutation

25 repeats of BoxB sequence

Promoter

CMV

Available Since

Nov. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pT2SK

Plasmid

#59383

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-kan

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1(S94A)

Plasmid

#59145

Purpose

YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

S94A

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

nEF

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Mutation

n/a

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV33 L1L2

Plasmid

#52493

Purpose

Expresses HPV33 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV33 L1
HPV33 L2

Expression

Mammalian

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGolden-Neo

Plasmid

#51422

Purpose

Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

PGK1 phosphoglycerate kinase 1 (PGK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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MR015-H11-R2-TALEN

Plasmid

#51555

Purpose

Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

H11-R2 TALEN

Use

TALEN

Expression

Mammalian

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-hEPO

Plasmid

#50436

Purpose

express human erythropoietin using lentiviral delivery system

Depositor

Juan Melero-Martin

Article

Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

Insert

EPO (EPO Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
pQF

Plasmid

#48095

Purpose

Cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQ5

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-hLIN28A

Plasmid

#47902

Purpose

Expresses human LIN28A in mammalian cells using retrovirus system.

Depositor

Shinya Yamanaka

Article

Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

human LIN28A (LIN28A Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2013

Availability

Academic Institutions and Nonprofits only

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a1Ic-HE3-pcDNA3

Plasmid

#45810

Depositor

Edward Perez-Reyes

Article

Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Insert

CACNA1I (CACNA1I Human)

Use

Making crna for oocyte injections

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Klb-EGFP

Plasmid

#45531

Depositor

Zipora Yablonka-Reuveni

Article

Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

Insert

beta Klotho (Klb Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2013

Availability

Academic Institutions and Nonprofits only

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N114 nLV EF-1a-Gal4-VP16-PGK-Puro

Plasmid

#44014

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Gal4/DBD (1-147)

Use

Lentiviral

Tags

VP16/Activation/Domain

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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