We narrowed to 42,149 results for: Eras

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KCC2G_HUMAN_D0

Plasmid

#79711

Purpose

This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2G (CAMK2G Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pD2_miniluc_newP7

Plasmid

#174105

Purpose

adding the mini luciferase and mini promoter to the MPRA lenti construct

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Insert

mini luciferase mini-promoter

Expression

Bacterial

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD

Plasmid

#179407

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD (Mecp2 Mouse)

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 2+linker3

Plasmid

#176920

Purpose

express Granulin 2 with linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2+linker3 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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KC1D_HUMAN_D0

Plasmid

#79723

Purpose

This plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1D (CSNK1D Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLV Myc-LBR

Plasmid

#175102

Purpose

Lentiviral expression of Myc-tagged mouse Lbr

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Lbr (Lbr Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_deltaLC

Plasmid

#98664

Purpose

Expresses MBP-tagged hnRNPA2 without the LC domain

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

G190Stop

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)

Plasmid

#75412

Purpose

Module for the expression of the Renilla Luciferase with the silencing suppressor P19

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

Renilla / P19

Use

Luciferase and Synthetic Biology

Expression

Plant

Promoter

35S

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAVA-LGF2(fs)-Gal11p(fs)

Plasmid

#127483

Purpose

Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Insert

LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)

Expression

Bacterial

Mutation

One nucleotide inserted between lambda CI and LGF…

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

Plasmid

#71214

Purpose

dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Tags

dSpCas9-BFP-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-miRFP670-Cry2WT

Plasmid

#122441

Purpose

Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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CDKL1_HUMAN_D0

Plasmid

#79699

Purpose

This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDKL1 (CDKL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Sumo3 PolQM1 deltains1

Plasmid

#78461

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2149-2170; Q2513R (please see…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
PB-TA-ERP2-hcGAS(R255A)

Plasmid

#208397

Purpose

To generate stable cell line expressing human cGAS(R255A) using PiggyBac transposon system

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human cGAS (CGAS Human)

Use

Piggybac transposon

Expression

Mammalian

Mutation

R255A

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_D290V

Plasmid

#98663

Purpose

Expresses MBP-tagged full length hnRNPA2 with D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

D290V

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Paragranulin-no linker

Plasmid

#176917

Purpose

express Paragranulin without linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA7-mVenus

Plasmid

#185647

Purpose

NDUFA7 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA7 (NDUFA7 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1 deltains2

Plasmid

#78460

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2264-2315; Q2513R (see deposi…

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
MBP-hnRNPA2_FL_P298L

Plasmid

#104468

Purpose

express MBP-tagged full length hnRNPA2 P298L

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

P298L

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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